摘要
目的:研究乌头碱(AC)在豚鼠和小鼠体外肝微粒体中代谢产物的差异。方法:优化2个种属肝微粒体与AC的P450反应体系,应用高效液相色谱-高分辨质谱联用(HPLC-HRMS)和超高相液相色谱-多级质谱(UPLC-MS/MS)法测定AC在2种肝微粒体中的细胞色素氧化酶(CYP)代谢产物。结果:AC在2种肝微粒体中均代谢产生8种代谢产物,其中6种为CYP代谢产物;脱甲基、脱乙基、脱氢和氧化反应是其主要代谢特征。结论:乌头碱在豚鼠和小鼠体外肝微粒体中的CYP主要代谢途径相同。
OBJECTIVE：To study the difference of the metabolites of aconitine（AC） in liver microsomes of guinea pig and mice.METHODS：HPLC-HRMS and UPLC-MS/MS were developed to determine the metabolites of AC in two liver microsomes,after optimizing the cytochrome P450 reaction systems of AC in two liver microsomes.RESULTS：Eight metabolites were detected in both two reaction systems,and six of them was determined as CYP-mediated metabolites.The metabolic pathways were demethylation,de-ethylation,dehydrogenation and oxidation reaction.CONCLUSION：There was no species different in the metabolic pathways of AC in liver microsomes of guinea pig and mice.
出处
《中国药房》
CAS
CSCD
2012年第7期590-593,共4页
China Pharmacy
