丁香酚在大鼠体内的组织分布研究

Study on Tissue Distribution of Eugenol in Rats

目的:研究丁香酚在大鼠体内的组织分布规律。方法:大鼠ig给予丁香酚后,应用高效液相色谱(HPLC)法测定丁香酚在大鼠心、肝、脾、肺、肾、胃、大肠、小肠、脑、前列腺中的含量。色谱柱为AllsphereODS-2RP-18(250mm×4.6mm,5μm),流动相为甲醇:0.5%冰醋酸=63:37(V/V,pH3.80),检测波长为280nm,柱温为30℃,流速为0.8mL.min-1。结果:丁香酚在大鼠心、肝、脾、肺、肾、胃、大肠、小肠、脑、前列腺的线性回归方程分别为A=185.87c-0.3951(0.9972)、A=174.63c-0.1013(0.9992)、A=164.5c+0.0174(0.9975)、A=191.98c-0.6649(0.9912)、A=192.34c-0.0897(0.9878)、A=180.26c+0.1690(0.9983)、A=146.29c+0.1356(0.9993)、A=159.07c+0.2213(0.9991)、A=158.58c-0.0504(0.9987)、A=97.218c+1.6503(0.9335)。丁香酚组织样品匀浆液浓度在0.011～2.158μg.mL-1范围内与峰面积比值呈良好线性关系。最低检测浓度为0.011μg.mL-1。结论:大鼠ig给药后,较快的分配到各脏器组织中,分布范围广,其中以肠、脾中水平较高,脑中较低。

OBJECTIVE：To study the tissue distribution regularity of eugenol in rats.METHODS：HPLC method was adopted to determine the contents of eugenol in heart,liver,spleen,lung,kidney,stomach,small intestines,large intestines,brain,prostate,respectively after intragastrical administration of eugenol in rats.Allsphere ODS-2 RP-18 column was used with mobile phase consisted of methanol-0.5%acetic acid（63：37）,（V：V,pH 3.80） at the flow rate of 0.8 mL·min-1.The detection wavelength was set at 280 nm,and column temperature was 30℃.RESULTS：The linear regression equations of eugenol in above tissue were A=185.87c-0.395 1（0.997 2）,A=174.63c-0.101 3（0.999 2）,A=164.5c＋0.017 4（0.9975）,A=191.98c-0.664 9（0.991 2）,A=192.34c-0.089 7（0.987 8）,A=180.26c＋0.169 0（0.998 3）,A=146.29c＋0.135 6（0.999 3）,A=159.07c＋0.221 3（0.999 1）,A=158.58C-0.050 4（0.998 7）,A=97.218c＋1.650 3（0.933 5）,respectively.The linear range of eugenol in homogenate was 0.011～2.158 μg·mL-1 and the lowest detection limut of gentianine was 0.011 μg·mL-1.CONCLUSION：After intragastrical administration,it spread to each viscera and tissue rapidly and widely.The level of it is higher in intestines and spleen,and lower in brain among them.