HPLC法测定柘木糖浆中山柰酚的含量

Content Determination of Kaempferol in Zhemu Syrup by HPLC

目的:建立测定柘木糖浆中山柰酚含量的方法。方法:采用高效液相色谱法。色谱柱为CalesilODS-100C18(250mm×4.6mm,5μm),流动相为甲醇-0.2%乙酸溶液(梯度洗脱),流速为1mL.min-1,检测波长为360nm。结果:山柰酚进样浓度在1～50μg.mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均加样回收率为99.77%,RSD=1.21%(n=9)。结论:本方法快速、简便、专属性强,可用于柘木糖浆的质量控制。

OBJECTIVE：To establish a method for the content determination of kaempferol in Zhemu syrup.METHODS：HPLC method was adopted.The determination was performed on Calesil ODS-100 C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-0.1% acetic acid（gradient elution）at a flow rate of 1 mL·min-1.The detection wavelength was set at 360 nm.RESULTS：The linear range of kaempferol was 1～50 μg·mL-1（r=0.999 8）.The average recovery rate was 99.77%（RSD=1.21%,n=9）.CONCLUSION：The method is rapid,simple and specific,and it can be used for the quality control of Zhemu syrup.