东莞市2010年新型H1N1流感病毒实验结果分析

Analysis on the experimental result of influenza A/H1N1 (2009) virus in Dongguan city in 2010

目的:了解东莞市2010年新型H1N1流感病毒的流行情况及疫苗免疫效果,为新型H1N1流感病毒的防控提供依据。方法:采集临床疑似流感病例的咽拭子分泌物,用荧光定量RT-PCR方法检测核酸。采集接种新型H1N1疫苗前后双份血清,用血凝抑制实验检测新型H1N1抗体滴度。结果:全年共检测1811份样品,新型H1N1阳性103例,阳性率为5.39%,男女之间的检测阳性率有显著性差异,女性高于男性(χ2=4.351,P=0.037)。病例数最多的年龄组为15岁～24岁和25岁～40岁,分别为32例和26例。发病高峰在1月份,阳性病例数为58份,占全年总阳性例数的56.31%。疫苗接种前后,几何平均滴度(GMT)增长14.09倍,免疫成功率为76.32%。结论:2010年东莞市新型H1N1流感病毒流行情况较2009年发生较大的改变,新型H1N1流感疫苗有很好的免疫效果,能使大部分接种对象产生有效的抗体。

Objective：To understand the epidemic situation of influenza A/H1 N1 （2009） virus and the immune effect of the vaccine in Dongguan city, and provide scientific basis for the prevention and control of influenza A/H1 N1 （2009）virus. Methods： The throat swabs of doubtful were sampled to detect the nucleic acid by RT - PCR. Paired serum was collected before and after inoculating vaccine and the antibody titer of neotype A H1N1 was detected by hemagglutination inhibition test. Results： Total 1811 specimens were detected, 103 specimens were positive, positive rate was 5.39%, there was significant difference between the positive rate of male and female （X2 = 4. 351, P = 0. 037）. There were 32 and 26 cases respectively in the 15 -24 and 25 - 40 age groups which had the most cases among all age groups. The peak time of incidence was January,the number of positive cases was 58, accounting for 56.31% of the total positive cases in 2010. The GMT increased 14.09 times after vaccine inoc- ulation, immune achievement ratio was 76.32%. Conclusion： The epidemic situation of influenza A/H1N1 （2009） virus in Dongguan city in 2010 has changed against the epidemic situation in 2009, the influenza A/H1 N1 （2009） virus vaccine can cause most objects of inoculation to produce effective antibody.