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T-RFLP技术在人体肠道菌群研究中的应用进展

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摘要 微生物群落的多样性一直是微生态学和环境微生物学研究的重点，在人类疾病的预防和治疗及机制研究中占据着举足轻重的地位。随着分子生物学技术的迅速发展，人们不再满足单纯的依赖传统的培养及镜检方法对微生态进行研究，而越来越多的是从分子水平的角度去研究复杂的微生物群落组成及其多样性。
作者 熊鹿言 余倩 唐海丰
机构地区 上海市普陀区疾病预防控制中心 四川大学公共卫生学院
出处 《中国卫生检验杂志》 CAS 北大核心 2012年第1期191-193,共3页 Chinese Journal of Health Laboratory Technology
关键词 T—RFLP 多样性 肠道菌群
分类号 R446.5 [医药卫生—诊断学]
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参考文献31

  • 1Nagashima K, Hisada T, Sato M,et al. Application of New Primer- Enzyme ombinations to Terminal Restriction Fragment Length Polymor- phism Profiling of Bacterial Populations in Human Feces [ J ]. Appl Environ Mierob,2003,69 (2) : 1251 - 1262.
  • 2Matsumoto M, Sakamoto M, Hayashi H. Novel phylogenetic assignment database for terminal - restr/ction fragment length polymorphism analy- sis of human colonic microbiota [ J]. J Microbiol Methods,2005,61 (3) :305 -319.
  • 3Li F, Hullar MA, Lampe JW. Optimization of terminal restriction frag- ment polymorphism (T -RFLP) analysis of human gut microbiota[ J ]. J Microbial Methods, 2007,68 (2) :303 - 311.
  • 4Antonia S, Regis B, Malene S, et al. Direct Analysis of Genes Encoding 16S rRNA from Complex Communities Reveals Many Novel Molecular Species within the Human Gut[ J]. Appl Environ Microb, 1999,65 ( 11 ) :4799 -4807.
  • 5Sakata S, Tonooka T, Ishizeki S, et al. Culture - independent analysis of fecal microbiota in infants, with special reference to Bifidobactcrinm species [ J ]. Ferns Microbial Lett,2005,243 ( 2 ) :417 - 423.
  • 6Wang M, Ahere S, Antonsso M, et al. T - RFLP combined with princi- pal component analysis and 16S rRNA gene sequencing: an effective strategy for comparison of fecalmierobiota in infants of different ages [ J ]. J Microbiol Methods,2004,59 ( 1 ) : 53 - 69.
  • 7Dicksved J, Floistrup H, et al. Molecular fingerprinting of the fecal mi- crobiota of children raised according to different lifestyles [ J ]. Appl Environ Microb,2007,73 (7) :2284 - 2289.
  • 8Hayashi H, Sakamoto M, Kitaha M, Benno Y, et al. Molecular Anal- ysis of Fecal Microbiota in Elderly Individuals Using 16s rRNA Library and T - RFLP [ J ], MierobioI Immuna1,2003,47 ( 8 ) :557 - 570.
  • 9Hayashi H,Takahashi R,Nishi T, et al. Molecular analysis of jejunal, ileal, caecal and recto -sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymor- phism [ J ]. J Med Microbiol,2005,54 ( 11 ) : 1093 - 1101.
  • 10F Layer, B Ghebremedhin, K -A Moder, et al. Comparative study u- sing various methods for identification of Staphylococcus species in clinical specimens[ J]. J Clin Microbio1,2006 ,dd( 8 ) :2824 - 2830.

二级参考文献3

  • 1Amann R, Fuchs BM, Behrens S. The identification of micro - organisms by fluorescence in situ hybfidisatio.Curr.Opin.Biotechnol, 2001, 12:231-236.
  • 2Kawai M, et al. 16S ribosomal DNA - based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient geleletrophoresis. Appl Environ Microbiol, 2002,68 (2): 699-704.
  • 3Christensen JE, Stencil JA, Reed KD. Rapid identification of bacteria from positive blood cultures by terminal restriction fragment length polymorphism profile analysis of the 16S rRNA gene.J Clinical Microblol, 2003,41 (8): 3790-3800.

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中国卫生检验杂志
2012年 第1期

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