静磁场作用下骨骼肌细胞形态学变化的研究

Study of morphological changes in rats maxillofacial skeletal muscle cells after exposed in static magnetic fields

目的研究静磁场对大鼠面颌骨骼肌细胞生长形态的影响以及细胞数量的变化。方法将传代培养的SD大鼠面颌骨骼肌细胞分别加磁,强度为180、280、360mT,作用时间为12、36、60h,照相记录观察细胞形态的变化。结果 180mT和280mT磁场强度对细胞有明显的促增值作用,细胞形态正常;360mT的磁场强度在36h之内对细胞的增殖作用较明显,作用至60h时,部分细胞发生坏死,细胞形态由梭形变成无规则的悬浮细胞。结论静磁场对大鼠面颌骨骼肌细胞具有明显的促进增殖作用,且细胞的损伤程度与磁场强度和作用时间成正相关关系。

Objective To observe the morphological and numerary changes of skeletal muscle cells after exposed different density SMF（static magnetic fields）.Methods The cultured skeletal muscle cells were exposed in the static magnetic fields for 12,36,60 h respectively.The skeletal muscle cell morphological changes were observed by taking photo.Results In 180mT group and 280mT group,cells significantly increased;In 360 mT group,cells grown a good condition before 36 h,but in 60 h,cells apoptosis was obviously observed.Conclusion Static magnetic fields intensity can promote cell proliferation in certain range,but high-intensity magnetic field（ 360 mT） maybe is harmful to tissue health.