氟化钠亚慢性染毒对雌性小鼠氧化损伤的研究

Sodium fluoride subchronic poison impact female mice estrogen receptor expression

目的研究氟化钠亚慢性染毒对雌性小鼠所产生的氧化损伤。方法取雌性小鼠40只,随机分成4组,每组10只。即对照组,氟低剂量组(1 mg/L),氟中剂量组(5 mg/L)、氟高剂量组(25 mg/L)。经饮用水染毒3个月。计算脏器系数、测量骨组织氟浓度,用试剂盒测定血清中血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果染毒过程中各组之间体重差异无统计学意义(P>0.05)。肝脏脏器系数从高到低依次为对照组、氟低剂量组、氟中剂量组和氟高剂量组(46.56±2.46)、(47.03±6.25)、(45.84±5.90)、(42.19±3.89),氟高剂量组与对照组相比显著下降,差异有统计学意义(P<0.05),其余各脏器系数变化无统计学意义(P>0.05)。中、高剂量(5.46±0.26)、(5.46±0.33)染氟组股骨脏器系数显著增加,与对照组(4.98±0.25)比较,差异有统计学意义(P<0.05)。染毒后骨组织中氟浓度显著升高,从低到高依次为对照组、低浓度组、中浓度组和高浓度组(711.37±263.83)、(998.75±254.87)、(1 195.21±159.73)、(1 957.00±273.19)μg/g,其中,中、高染氟组与对照组比较,差异有统计学意义(P<0.05)。氟高剂量组SOD活性(0.77±0.03)U/ml与对照组(0.86±0.07)U/ml比较显著降低(P﹤0.05);氟低剂量组、氟中剂量组、氟高剂量组(7.61±0.86)、(8.65±1.02)、(8.75±1.47)nmol/ml与对照组(4.36±0.36)nmol/ml比较MDA活性均升高,差异有统计学意义(P﹤0.05)。结论染毒后血清中SOD活力下降,各组MDA水平显著升高,提示存在氧化应激损伤。

Objective To study the subchronic toxicity of sodium fluoride on the estrogen receptor expression level of female mice. Method 40 ICR mice/female, weight 18 to 20 g ）were randomly divided into 4 groups, 10 in each group, and exposed to sodium fluoride solution （0 mg/L ,1 mg/L 5 rag/L, 25 rag/L） from drinking water for 3 months. The fluorine concentrations in bone tissues were detected by fluorine ion selective electrode. The expression levels of ERct, ERβ, IL - 6 mRNA were deter- mined by RT - PCR method. Results Weight differences between groups were not statistically significant （ P 〉 0.05 ） . The organ coefficient of liver in control group was the highest, followed by low - dose group, medium - dose group and high - dose group （ 46.56 ± 2.46 ）, （ 47.03 ± 6.25 ） , （ 45.84 ±5.90 ）, （ 42.19 ± 3.89 ）. Compared with control group （ 4.98± 0.25 ） , the organ coefficients of bone in medium - dose and high - dose group （ 5.46± 0.26 ） , （5.46± 0.33 ） were significantly increased （ P 〈 0.05 ）. The fluorine concentrations were gradually increased, from control group to high - dose group （ 711.37 ± 263.83 ） , （998.75 ± 254.87 ） , （ 1195.21 ± 159.73 ） , （ 1957.00 ± 273.19 ）μg/g, and the differences between medium/high - dose group and control group were statistically significant （ P 〈 0.05）. RT - RCR results showed that estrogen receptor expression levels in ovarian tissues decrease with increased dose, and the difference between high -dose group and control group about the expression levels of ERα was statistically significant （ P 〈 0.05 ）. Conclusions Fluoride can decrease the expression of estro- gen receptor in ovarian tissue, and maybe affect the animal reproductive development.