逐次加深模拟氦氧饱和潜水对潜水员细胞免疫状态的影响

Effects of gradational simulated saturation diving on cellular immune function of the divers

目的 探讨深度到由浅到深逐次进行模拟氦氧饱和潜水对潜水员细胞免疫状态的影响.方法 9名健康男性潜水员逐次由浅至深进行65、250、480 m氦氧饱和暴露,每次暴露前后采集抗凝血,经流式细胞术检测外周血T淋巴细胞亚群的变化.结果 在250 m饱和暴露后外周血CD4淋巴细胞亚群和CD4/CD8（41.26±8.46和1.36±0.50）较暴露前（35.63±7.93和1.04±0.36）显著升高；65 m及480 m饱和暴露前后,外周血淋巴细胞亚群未发生明显改变.未经65m饱和暴露实验的潜水员,在480m饱和暴露阶段,较其他潜水员的免疫状态更容易受到影响.结论 逐次加大深度氦氧饱和暴露可以防止饱和潜水环境中潜水员细胞免疫功能的抑制.

Objective To investigate the effects of gradational simulated saturation diving on the cellular immune function of the divers.Methods Nine male healthy divers conducted simulated saturation diving gradationally at 65 m,250 m and 480 m.Blood samples were collected before and after each pressure exposure to detect changes in T lymphocyte subgroups of the peripheral blood,with flow cytometry.Results The level of CD4 lymphocytes and CD4/CD8 （41.26 ± 8.46、1.36 ± 0.50 ） ratio increased significantly following 250 m saturation exposure,when compared with those before dive （ 35.63 ± 7.93、1.04 ± 0.36）.However,no significant changes were detected in CD4 lymphocytes of the peripheral blood,both before and after saturation exposure at 65 m and 480 m.For those divers who didn ＇t experience the 65 m saturation exposure,their immune function during the stage of the 480 m saturation exposure was more easily affected,when compared with that of other divers.Conclusions It seemed that gradational heliox hyperbaric exposure could protect the cellular immune function of saturation divers.