乌司他丁对羊多脏器功能障碍综合征的防护作用

Protective effects of ulinastain on multiple organ dysfunction syndrome in sheep models

目的探讨乌司他丁(UTI)对多脏器功能障碍综合征(MODS)的防治作用。方法 21只杜泊绵羊随机分为对照组、模型组、UTI治疗Ⅰ组和治疗Ⅱ组。模型组、治疗Ⅰ组和Ⅱ组静脉注射内毒素(6μg/kg),治疗Ⅰ组静脉注射UTI(30000U/kg),1次/d,连续3d,治疗Ⅱ组静脉注射UTI(30 000U/kg),2次/d,连续3d。各组分别于0,1,2,3,6和12h时间点测定动脉氧分压;于0,3,6,12,24,48和72h测定脏器生化指标天门冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)、肌酐(Cr)和血常规指标。结果与模型组比较,UTI治疗组的氧分压下降幅度小;治疗组AST,ALT,TBIL和Cr的升高明显低于模型组(P<0.05),治疗Ⅰ组和Ⅱ组之间各项指标改善无统计学差异;模型组、治疗Ⅰ组和Ⅱ组绵羊的死亡率分别为66.6%、50.0%和33.3%。结论预防性应用UTI能降低内毒素诱导的MODS模型绵羊的死亡率,提高动脉氧分压,减轻内毒素所致的肺、肝和肾等脏器的损伤,具有保护作用。

Objective To determine the protective effect of ulinastain（UTI） on multiple organ dysfunction syndrome（MODS）. Methods Sheep models of MODS were induced by lipopolysaccharide（LPS）.The animals were randomly divided into four groups： normal control group,MODS group,UTI treatment groupⅠand groupⅡ.The anaesthetized animals received intravenous infusion of LPS（6μg/kg） to induce MODS in MODS group and UTI treatment groups.The animals received intravenous injection of UTI （30000U/kg） for 3 days,once daily in UTI treatment groupⅠand twice daily in groupⅡ.Arterial oxygen pressure（PaO_2） was measured at 0（basement）,1,2,3,6 and 12h respectively.Blood samples were taken for determination of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,creatinine（Cr）,total bilirubin（TBIL） and blood routine at 0（basement）, 3,6,12,24,48 and 72h respectively.Results PaO_2 decreased as early as at 1 hour after injection of LPS.Compared with MODS group,PaO_2 decreased less significantly in UTI treatment group.AST,ALT,TBIL and Cr in UTI treatment groups were significantly lower than those in MODS group（P0.05）,but there was no obvious difference between UTI treatment groupⅠand groupⅡ.The animal mortality was 66.6%,50.0%and 33.3%in MODS group,UTI treatment groupⅠand groupⅡrespectively.Conclusion Prophylactic application of UTI could reduce the mortality of MODS animals induced by LPS.It has protective effects on lung, kidney and liver injuries in MODS animals.