IgAN肾小球硬化大鼠模型血/尿胱抑素C水平与肾纤维化的相关性研究

The Relationship Between Serum/urinary Cystatin C levels and Renal Fibrosis in IgAN Rats with Glomerular Sclerosis

目的:探讨IgAN肾小球硬化大鼠模型血/尿胱抑素C水平的变化情况及其与肾小球硬化和小管间质纤维化的相关性。方法:将16只SD雄性大鼠随机分为假手术组和复合感染合5/6肾切除组,采用ELISA法检测大鼠第0、4、8、12周的血清胱抑素C水平及第0、4、6、8、12周的尿胱抑素C水平的动态变化,免疫荧光观察肾小球IgA表达情况,Masson染色观察第12周大鼠IgAN肾小球硬化和肾小管间质纤维化的情况,并进行肾小球硬化积分和肾小管-间质纤维化积分,分析其与血/尿胱抑素C水平变化的相关性。结果:复合感染合5/6肾切除组血/尿胱抑素水平均明显高于假手术组,呈时间依赖性上升,并与肾小球硬化积分和肾小管间质纤维化积分呈正相关(R1=0.81,R2=0.66,P<0.01和R3=0.70,R4=0.69,P<0.01)。结论:血/尿胱抑素C水平可能是反映IgAN肾小球和肾小管间质纤维化程度的可靠指标。

Objective：To investigate serum / urinary cystatin C levels and their correlation with glomerular sclerosis and tubulointerstitial fibrosis in IgAN rats with glomerular sclerosis. Methods：16 male SD rats were randomly divided into sham operation group and co-infectionwiht 5/6 nephrectomy group. Serum cystatin C levels at 0,4,8,12 weeks and urinary cystatin C levels at 0,4,6,8,12 weeks were detected by ELISA. Immunofluorescence was employed to assay glomerular IgA expression, and Masson staining was used to analyse glomerular sclerosis and tubulointerstitial fibrosis at 12 weeks. Moreover, The glomerular sclerosis and tubulointerstitial fibrosis were scored semiquantitatively by staining kidney section with Masson. The correlation between serum / urinary cystatin C levels and glomerular sclerosis/ tubulointerstitial fibrosis scores were analysized by Pearson correlation. Results：Serum/urinary cystatin C levels in co-infected with 5/6 nephrectomy group were significantly higher than the sham group, showed a time-dependent increase, were positively correlated with the glomerular sclerosis scores and tubulointerstitial fibrosis scores （R1=0.81,R2=0.66,P0.01 and R3=0.70,R4=0.69,P0.01）,respectively. Conclusion：Serum / urinary cystatin C levels may be reliable markers to reflect glomerular and tubulointerstitial fibrosis in IgA nephropathy.