细胞间黏附分子-1在自发性高血压大鼠肾损害作用的研究

Effects of Intercellular Adhesion Molecule-1 on Renal Damage in Spontaneously Hypertensive Rats

目的:研究细胞间黏附分子-1(ICAM-1)在自发性高血压大鼠(SHR)肾组织的表达及其与肾损害的关系。方法:以同龄雄性正常血压大鼠(WKY)和SHR为研究对象,分别于10周龄和28周龄检测两种大鼠尾动脉压、24h尿蛋白定量、肾功能等;留取肾组织行HE染色、免疫组织化学及RT-PCR法检测ICAM-1蛋白及mRNA表达情况,并作分析。结果:与同龄WKY大鼠比较,SHR尾动脉压和24h尿蛋白定量明显增高(P<0.05和P<0.05);与12周龄SHR比较,28周龄SHR尾动脉压和24h尿蛋白定量明显增加(P<0.05和P<0.05)。SHR组肾组织ICAM-1蛋白及mRNA表达较同龄WKY增强(P<0.05),28周龄SHR较10周龄SHR表达增强(P<0.05),ICAM-1表达与24h尿蛋白定量呈正相关(P<0.05)。结论:SHR出现蛋白尿时,肾组织ICAM-1表达增强,炎症参与了高血压肾损害的发生。

Objective：To study the expression of ntercellular adhesion molecule-1 （ICAM-1） in the spontaneously hypertensive rats （SHR） kidneys and the relationship between the level of ICAM-1 and renal damage. Methods：The arteria caudilis pressure, renal function and 24 h urinary protein of SHR and WKY rats were measured at the 10 th and 28 th weeks. The renal tissue specimens were taken for HE staining and the expressions of ICAM-1 in the kidney were examined by immunohistochemistry and RT-PCR. Results：Compared with the same age WKY rats, arteria caudilis pressure and 24-hour urinary protein in SHR significantly increased （P0.05和P0.05）. In SHR, the arteria caudilis pressure and 24 h urine protein at 28th week were significantly higher than that at 10 th week （P0.05和P0.05）. The ICAM-1 mRNA and protein expressions in SHR renal tissues significantly increased compared with same age WKY rats（P0.05）. Futher more, the ICAM-1 mRNA and protein expressions in SHR renal tissues at the 24 th weeks significantly increased compared with that at the 24 th weeks（P0.05）. The expression of ICAM-l was positively correlated with 24 h urine protein （P0.05）. Conclusion：The expressions of ICAM-1 in renal tissue increased in SHR with proteinuria. inflammation may be involved in the hypertensive renal damage.