非抑制电导检测离子色谱法测定硅藻培养液中硅酸根

Determination of Silicate in Cyclotella Meneghiniana Kiits Solution Using Non-suppressed Conductance Detection Ion Chromatography

采用戴安阴离子AS23(4 mm×250 mm)分析柱和AG23(4 mm×50 mm)保护柱、恒温电导检测器,建立了测定硅藻培养液中硅酸根(SiO32-)含量的非抑制电导检测离子色谱法。以4 mmol/L碳酸钠为淋洗液,淋洗液流速1.0 mL/min,进样体积100μL,采用峰高定量。该法测定SiO32-的线性范围为0～40 mg/L,检出限为0.017 mg/L,重复测定同一标样的相对标准偏差(RSD,n=7)小于5%,峰面积和峰高的RSD分别为10.9%、4.8%。测定不同生长时期硅藻培养液中的SiO23-,样品的加标回收率为102%～120%。该法灵敏、准确、简便易行,适用于硅藻培养液中SiO32-的检测。

A novel method was developed for the determination of SiO2-3 in Cyclotella meneghiniana Kiits solution by non-suppressed conductance detection ion chromatography.The chromatography was performed on a Dionex anion AS23（4 mm×250 mm） analytical column and AG23（4 mm×50 mm） guard column with a constant temperature conductance detector.The experimental conditions were optimized,and the optimal chromatographic conditions were using 4 mmol/L sodium carbonate as eluent with a eluent speed of 1.0 mL/min,injection sample volume of 100 μL.The calibration curve of silicate was linear in the range of 0-40 mg/L.The detection limit was 0.017 mg/L.The relative standard deviation（RSD,n=7） for standard solution was lower than 5%.The RSDs for peak area and peak height measurements were 10.9% and 4.8%,respectively.The proposed method was applied in the determination of silicate in Cyclotella meneghiniana Kiits solution in different growing periods,and the spiked recoveries were in the range of 102%-120%.With the advantages of simplicity,good sensitivity,low cost,the method was suitable for the determination of silicate in cyclotella meneghiniana kiits solution.