PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析

Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE

采用生物滴滤塔能够有效去除含苯乙烯恶臭气体,塔内微生物中含有大量的球菌和杆状菌。采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术研究处理苯乙烯恶臭气体的生物滴滤塔填料表面的微生物,结果表明,去除苯乙烯生物滴滤塔中有5种菌为降解苯乙烯的优势菌种;通过16S rDNA基因扩增测序同源性比对,结果显示嗜甲基杆菌属(methylophilus)丰度为50.5%,2种变形菌属(alpha proteobacterium、delta proteobacterium)相对丰度分别为16.9%和11.6%。

Styrene removal could be achieved with high efficiency in the biotrickling filter packed with ceramsite.There are a large number of coccus and rod bacterial in the biotrickling filter.The microbial community structures in the biotrickling filter for styrene removal were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE）.Based on 16S rDNA sequence data,results show that the predominant bacterias for degradation of styrene are methylophilus,alpha proteobacterium,delta proteobacterium,two uncultured bacteriums.The dominant bacteria,methylophilus,takes up 50.5%;while alpha proteobacterium and delta proteobacterium are take up 16.9% and 11.6%,respectively.