磁性c-erbB2反义探针对SK-Br-3肿瘤细胞株形态及表达的影响

Effects of magnetic c-erbB2 antisense probe on morphology and expression of SK-Br-3 cancer cell lines in vitro

目的:探讨磁性c-erbB2反义寡脱氧核苷酸探针对SK-Br-3肿瘤细胞株形态及表达的影响,阐明该探针是否同时具有诊断与治疗功能。方法:磁性c-erbB2反义探针转染高表达c-erbB2的SK-Br-3肿瘤细胞作为实验组,磁性c-erbB2反义探针转染Fuda肿瘤细胞株、SPIO及ASODN转染SK-Br-3肿瘤细胞、未转染的SK-Br-3肿瘤细胞设置为4个对照组,普鲁士蓝染色光镜观察细胞内铁的分布,透射电镜观察其超微结构,并检测细胞cerbB2蛋白表达变化及铁含量,行体外MR成像。结果:光镜显示SK-Br-3肿瘤细胞胞浆内散在分布铁颗粒,透射电镜观察到SK-Br-3肿瘤细胞胞浆内铁颗粒、团状吞饮体,细胞出现胞膜空泡化、细胞固缩、核固缩及凋亡小体,而对照组细胞无上述改变;与对照组比较,实验组SK-Br3细胞蛋白的表达抑制率明显升高,细胞铁含量增加(P<0.01),MR扫描显示信号减低(P<0.05)。结论:磁性c-erbB2反义探针能够顺利进入SK-Br-3肿瘤细胞,不仅能导致SK-Br-3细胞的凋亡,还能够被体外MR成像所检测,可能成为一种具有治疗与诊断意义的双功能探针。

Objective To observe the effects of the magnetic c-erbB2 antisense oligodeoxynucleotide probe on morphology and expression of SK-Br-3 cancer cells in vitro,and to discuss whether the probe has the functions of diagnosis and treatment.Methods SK-Br-3 cancer cells were transfected by magnetic c-erbB2 antisense probe as experiment group,Fuda tumor cells were transfected by magnetic c-erbB2 antisense probe,SK-Br-3 cancer cells were transfected by SPIO and ASODN,untransfected SK-Br-3 cancer cells as 4 control groups.The iron distribution in the transfected cancer cells was observed by Prussian blue staining,the ultrastructure was observed by transmission electron microscope,the protein expression of cerbB2 and the iron content were detected and MR imaging was performed.Results The iron particles scattered in the cytoplasm of transfected SK-Br-3 cancer cells under light microscope.The iron particles,groups-like pinocytosis,cell membrane vacuolization,cell shrinkage,nuclear condensation,and apoptotic bodies were found under transmission electron microscope,but the cells in control groups had no the changes mentioned above.Compared with control groups,the inhibitory rate of protein expression of SK-Br-3 cells was significantly increased,the iron content of cells was increased（P0.05）,the signal in MR imaging was decreased.Conclusion Magnetic c-erbB2 antisense probe can smoothly go into the SK-Br-3 cancer cells,it not only results in SK-Br-3 cell apoptosis but also can be found by MR imaging,it may become a bifunctional probe with significance in treatment and diagnosis.