大蒜多糖对慢性酒精中毒小鼠肝损伤的保护作用

Protective effects of garlic polysaccharide on liver injury in mice with chronic alcoholism

目的:通过大蒜多糖对小鼠酒精性肝损伤的干预效果研究,阐明其对慢性酒精中毒小鼠肝脏损伤的保护作用及机理,为研制开发防治酒精性肝病的天然抗氧化药物提供实验依据。方法:70只昆明小鼠随机分为对照组、模型组以及大蒜多糖低、中、高剂量(100、150和200mg.kg-1.d-1)组,每组14只,采取剂量递增法,用56°白酒建立小鼠慢性酒精中毒模型,同时用大蒜多糖进行干预。实验8周后,测定小鼠体质量、肝质量指数、肝脏丙二醛(MDA)和谷胱甘肽(GSH)水平、检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、Na+-K+-ATP酶、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的活性,光镜下观察小鼠肝脏组织形态学改变。结果:与对照组比较,模型组小鼠体质量明显下降(P<0.05),肝质量指数、血清ALT和AST活性及肝脏MDA水平均明显增高(P<0.05),Na+-K+-ATP、GSH-Px和SOD活性及GSH水平明显降低(P<0.05);肝脏出现明显脂肪空泡、炎细胞浸润等病理学改变。与模型组比较,大蒜多糖各剂量组小鼠肝质量指数、血清ALT和AST活性明显降低(P<0.05),GSH水平增加(P<0.05),GSH-Px和SOD活性明显增高(P<0.05);高、中剂量组小鼠体质量和Na+-K+-ATP活性明显增加(P<0.05),MDA水平明显降低(P<0.05),肝组织病变减轻。结论:持续过量饮酒可导致小鼠酒精中毒并对肝脏产生严重损伤;大蒜多糖对小鼠酒精性肝损伤有一定的保护作用,机理与其增强机体抗氧化能力有关。

Objective To study the interference effect of garlic polysaccharide on alcoholic liver injury in mice and clarify its protective effects and mechanism,and to provide experimental basis for developing natural antioxidant medicine for prevention and treatment of alcoholic liver disease liver.Methods 70 Kunming mice were randomly divided into normal group,control group,model group,garlic polysaccharide low,medium and high-dose（100,150,and 200 mg·kg-1·d-1）groups,fourteen mice in each group.Dose increasing method was used to set mouse chronic alcoholism models with 56°wine,meanwhile,the experiment was intervened with garlic polysaccharide.Eight weeks later,the weight,liver weight index,the levels of hepatic（MDA） and glutathione（GSH） and the activities of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,sodium potassium pump（Na＋-K＋-ATP）,glutathione peroxidase（GSH-Px）,superoxide dismutase（SOD） were measured,and the morphological changes of liver tissue in mice were observed with light microscope.Results Compared with control group,the weights of the mice in model group were markedly decreased（P0.05）,while the liver weight index,ALT and AST levels in serum,and MDA content in liver were all significantly increased（P0.05）;the activities of Na＋-K＋-ATP,GSH-Px and SOD and GSH level were evidently decreased（P0.05）.In addition,the pathological changes in liver appeared fat vacuole,inflammatory cell infiltrate,etc.Compared with model group,in garlic polysaccharide groups,the liver weight indexes of mice and the levels of serum ALT and AST were significantly decreased（P0.05）,while the levels of GSH were increased and the activities of GSH-Px and SOD were greatly increased（P0.05）;in medium and high dose groups,the weights and the activities of Na＋-K＋-ATP were significantly increased（P0.05）,while the levels of MDA were obviously decreased（P0.05） and the degree of pathological changes in liver tissue was reduced.Conclusion Unremitting superfluous drinking can lead mice to alcoholism,and cause serious damage to their livers.Garlic polysaccharide has certain protective effects on liver injury induced by alcohol in mice.The mechanism is relevant to its increasing anti-oxygen capacity of the organism.