治疗用卡介苗抑瘤小鼠模型的建立

Establishment of a mouse model for tumor suppression of therapeutic

目的建立治疗用卡介苗抑瘤小鼠模型，以研究治疗用卡介苗的抗肿瘤疗效。方法分别将适宜浓度的治疗用卡介苗溶液（20、10和5mg／ml）、环磷酰胺溶液（7．2mg／ml）和灭菌生理氯化钠溶液与适宜浓度的小鼠肝癌H22细胞（1×10^7个／ml）混合制成治疗用卡介苗处理液、阳性对照液和阴性对照液，皮下接种BALB／c小鼠，接种后4周，测定小鼠的肿瘤大小和重量，计算每组小鼠的平均肿瘤重量和瘤重抑制率，采用t检验对测定的结果进行统计学分析。结果与阴性对照组相比，10mg／ml治疗用卡介苗处理组的肿瘤重量明显减轻（t=3．41，P〈0．05），肿瘤体积明显缩小（t=4．88，P〈0．05），表明采用治疗用卡介苗处理可使肿瘤生长受到抑制。结论建立的治疗用卡介苗抑瘤小鼠模型可用于卡介苗的抗肿瘤疗效研究。

Objective To establish a mouse model for studying tumor suppression of therapeutic BCG （tBCG）. Methods tBCG treatment, positive control and negative control solutions were prepared by mixing tBCG （20, 10 and 5 mg/ml）, cyclophosphamide（7.2 mg/ml）and sterile sodium chloride solutions with mouse liver cancer H22 cell solution （1 × 10^7/ml）, respectively, and BALB/c mice were inoculated subcutaneously with a kind of mixed preparations. Tumor sizes and weights of mice were measured, and average weights and suppression rates of tumor were calculated. The statistical analysis of the results was made by t tset. Results Tumor weights and sizes in tBCG treatment group（ 10 mg/ml） reduced significantly as compared with those in negative group （ t = 3.41, P 〈 0. 05 ; t = 4. 88, P 〈 0. 05 ）. The results suggested tBCG treatment could suppress tumor growth. Conclusion The established mouse model for tumor suppression of tBCG can be used for studying antitumor effect of tBCG.