恩拉霉素微生物检定法的研究

The Research of Microbiological Assay for the Enramycin

以枯草芽孢杆菌为检定菌,检定培养基Ⅰ号为试验用培养基,采用双碟法进行恩拉霉素样品测定。通过浸提溶液组成、浸提时间、检定菌浓度考察,优化了检定条件。在此基础上,确立了恩拉霉素浓度的对数值与抑菌圈直径的线性范围,并对所建立的微生物检定法进行准确度、最小检出限等考察。结果表明,采用丙酮浸提液B、浸提恩拉霉素样品80min,恩拉霉素浸提较完全。在检定菌浓度106个/mL的条件下,恩拉霉素浓度在0.56～35.79U/mL之间,其浓度的对数值与相应的抑菌圈直径呈直线相关,相关系数r为0.9960。将建立的恩拉霉素微生物检定法用于恩拉霉素样品测定,回收率为95.90%,相对标准偏差(RSD)为1.95%。本方法可用于含恩拉霉素的样品测定。

Samples containing enramycin were determinated by the double disk method with Bacillus subtilis as test microorganism and antibiotics standardization medium Ⅱ.The determination conditions were optimized through the investigations of extraction solution composition,extraction time and the concentrations of Bacillus subtilis.The linearity of the dose-response relationship was analysised upon it,and the accuracy of the method was checked.The results showed that the optimum deternmination conditions were acidic acetone solution B and 80 min for extraction.The calibration curve was linear within the range from 0.56 to 35.79 U/mL between the logarithm of concentration and the diameter of inhibition zone with the 10^（6） spores /mL of test microorganism constration,the correlation coefficient was 0.9960.The proposed method has been applied to the determination of samples containing enramycin.The recoveries was 95.90%,relative standard deviation（RSD） was 1.95%.This method applies to the detection of enramycin sample.