中药添加剂对海南文昌鸡消化酶活性的影响

Study on Effect of Traditional Chinese Medicine Additive on Digestive Enzyme Activity of Hainan Wenchang Chicken

试验旨在研究中药添加剂对海南文昌鸡消化酶活性的影响。将180只雌性文昌鸡随机分为5组,每组3个重复,每个重复12只鸡。第1组为空白对照组,饲喂基础日粮;第2组为抗生素对照组,在基础日粮中添加600mg/kg的金霉素;第3、4、5组分别在基础日粮中添加0.1%、0.2%和0.3%的中药添加剂。试验共进行6周。每周称重、计量采食量。第35、56日龄时,每组分别取接近平均体重鸡6只,屠宰后迅速取胰腺和十二指肠内容物速冻,然后制备匀浆以测定胰腺蛋白酶活性和十二指肠内容物淀粉酶活性。结果显示,0.2%中药添加剂能显著提高日增重(P<0.05),0.2%的中药添加剂能够提高胰蛋白酶活力,但差异不显著(P>0.05),0.2%和0.3%的中药添加剂均能够显著提高肠道内容物中淀粉酶活力(P<0.05)。因此,中药添加剂能够提高文昌鸡胰蛋白酶和肠道淀粉酶活力,以0.2%的添加量较适宜。

To study the effect of traditional Chinese medicine additive（TCMA） on Wenchang chicken.180 female Wenchang chicken for a test of 6 weeks were randomly assigned to 5 groups with 3 replications each,each replication contain 12 chicken.Diets containing 0,600 mg aureomycin,0.1%,0.2% and 0.3% TCMA/kg were fed to group 1,group 2,goup 3,group 4 and group 5,respectively.Bodyweight and feed intake of chicken in same replication were measured per 7 days.At the age of 35 and 56 days,6 chicken with closed body weight from each group were slaughtered quickly to get pancreas and content of duodenum.Trypsinase activity and amylase activity of content of duodenum were measured.Results showed that,the daily weight gain of Wenchang chicken was improved significantly in the group containing 0.2% TCMA（P〈0.05）;trypsinase activity of the 0.2% TCMA group was improved（P〈0.05） and the amylase activity of content of duodenum was increased significantly both in 0.2% TCMA group and 0.3% TCMA group（P〈0.05）.Therefore,trypsinase activity and amylase activity of content of duodenum can be improved by TCMA,a dose of 0.2% of the TCMA was suitable for Wenchang chicken.