重组瘦蛋白对猪内分泌代谢和胴体品质的影响

Effects of Recombinant Leptin on Endocrine Metabolism of Porcine

本研究利用重组瘦蛋白进行动物饲养试验,研究了重组瘦蛋白对猪内分泌代谢和胴体品质的影响。结果表明,在腹腔注射0.5和1.0mg/kg体重重组瘦蛋白后,血清胰岛素含量从注射7d后开始降低,并且一直持续到试验结束,其中7～21d显著低于对照组,降低的幅度为12%～20%(P<0.05);血清T3含量在注射后7～14d和28～42d有显著提高(P<0.05);T4含量在注射后7～21d差异显著(P<0.05);GH含量在注射后7～28d显著高于对照组,提高的幅度为9%～25%(P<0.05);血清IGF-Ⅰ含量在注射后7～21d显著高于对照组,提高的幅度为9%～16%(P<0.05);同时,腹腔注射0.5和1.0mg/kg体重重组瘦蛋白与对照组相比,胴体瘦肉率提高6.70%和7.30%(P<0.05);脂肪率降低16.00%和18.75%(P<0.05);背膘厚降低18.14%和23.01%(P<0.05);板油重降低22.95%和24.59%(P<0.05)。可见,腹腔注射不同剂量的重组瘦蛋白后在一定时间内对猪内分泌有不同程度的调节作用,重组瘦蛋白可显著提高猪肉的瘦肉率,降低脂肪率、背膘厚和板油重,从而起到了调节猪胴体品质的作用。

In order to study the regulation of recombinant leptin on endocrine metabolism of swine, recombinant leptin were used in this feeding experiment. The results showed that endocrines metabolism of swine were regulated by injected recombinant leptin. Injecting recombinant leptin with 0.5 and 1.0 mg/kg body weight could regulate endocrines, content of serum insulin was remarkably reduced by 12% to 20% from the first to third week （P〈0.05）. Content of serum triiodothyronine（T3） were increased significantly from the first to the second week and the forth to sixth week （P〈0. 05）. Tetraiodothyronine （T4） were increased significantly from the first to the third week （P〈0.05）. Growth hormone（GH） were all higher than control group after injected with recombinant leptin, increased by 90/60 to 25% from the first to forth week remarkably （P〈0.05）. Content of serum insulin-like growth factor- 1 （IGF- 1 ） was higher than control group from the first to the third week and was significantly increased by 9% to 16% （P〈0.05）. Compared with control group, intraperitoneally injected with 0.5 and 1.0 mg/kg body weight recombinant leptin, the percentage of lean meat was increased 6.70% and 7.30% （P〈0.05）, the percentage of fat was decreased 16.00% and 18.75% （P〈0.05）, backfat thickness was decreased 18.14% and 23.01% （P〈0.05） and the leaf fat depot decreased 22.95% and 24.59% （P〈0, 05）. Carcass characteristics of swine were not significantly difference by intraperitoneally injected recombinant leptin with 0.1 mg/kg body weight（P〈0. 05）.