摘要
为检测卵泡内小肽的表达,采用基质辅助激光解离串联飞行时间质谱(MALDI-TOF/TOF MS)建立了成熟卵泡液的多肽表达模型,比较小卵泡(直径<4mm)、中等卵泡(直径4～8mm)和成熟卵泡(直径>8mm)的多肽表达变化。结果表明,3种形态的卵泡液存在12个显著差异多肽,其中在成熟卵泡内上调6个,下调有5个;在中等卵泡内上调4个,下调5个。其中分子质量约为1746u的多肽在成熟卵泡中特异性表达,可作为卵泡成熟的潜在生物标志物之一。
In order to detect the peptides expression profile in follicles,the matrix-assisted laser desorption ionization mass spectrometry was used to establish a model of peptide expression profile and compare expression changes of peptides from small follicles（（φ〈4 mm）,medium follicules（4 to 8 mm） and mature follicles（φ〉8 mm）.The results indicated that there were 12 peptides to express significantly different among three shapes of follicles.6 peptides increased and 5 peptides decreased in mature follicles.The 1746 u of peptide expressed specifically in mature follicles,which might be used as the one of the underlying biomarkers for follicles maturation.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第1期132-136,共5页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30860192)
广西科技厅基础条件平台建设(09-007-01)
高等学校博士点科研基金(20104501110001)
