城市污水厂活性污泥胞外聚合物的三维荧光特性分析

Three dimensional excitation-emission florescence spectra of expolymer substances in activated sludge of WWTP

对厦门5个城市污水处理厂活性污泥溶解性有机物SMP,松散型胞外聚合物LB-EPS和紧密结合型胞外聚合物TB-EPS的三维荧光光谱分析表明,SMP,LB-EPS和TB-EPS都有3个明显的特征峰,其中Peak A（Ex/Em=225~230/335~350nm）和Peak B（Ex/Em=275~280/330~350nm）都为类蛋白荧光物质,Peak C（Ex/Em=320~350/420~445nm）为腐殖酸类荧光物质.LB-EPS和TB-EPS荧光峰强度同其对应的TOC浓度有一定相关性.荧光强度综合指数FRI分析表明,微生物副产物更多的存在于TB-EPS中,其FRI总和同TOC浓度呈一定相关性.

Three-dimensional emission and excitation matrixs（EEMs） was used to characterize the soluble micro-products（SMP）,loosely-bound extracellular polymeric substances（LB-EPS） and tightly-bound extracellular polymeric substances（TB-EPS） extracted from activated sludge in five municipal wastewater treatment plants in Xiamen（WWTP）.Three fluorescence peaks were identified in EEMs of SMP,LB-EPS and TB-EPS,in which Peak A（Ex/Em=225~230/335~ 350nm） and Peak B（Ex/Em=275~280/330~350nm） were attributed to the protein-like fluorophores,and Peak C（Ex/Em=320~350/420~445nm） was characterized as humic-like fluorophores.Results of the peak intensity showed that the major components of LB-EPS and TB-EPS were significant correlated with TOC concentration in some degree.Fluorescence regional integration（FRI） analysis showed that microbial by-products existed in TB-EPS much more than that in LB-EPS and SMP.The sum FRI of I,II and IV regions presented linear relationship with TOC concentration in LB-EPS and TB-EPS.