鲁枣1号组织培养和植株再生体系研究

Study on Tissue Culture and Regeneration System of Jujube Variety Luzao 1

以鲁枣1号为试材,以正在生长的枣头嫩枝为外植体,进行了芽的诱导和试管苗快繁研究。结果表明,嫩枝茎段上未萌发的主芽在启动培养基(MS+1 mg/L BA+0.2 mg/L IBA+3%蔗糖)上可萌发成新梢,萌发率为54.8%。在相同的启动培养基上,远离培养基的茎段切口处可诱导产生不定芽,不定芽再生率为23.8%。不定芽和主芽新梢在MS+2 mg/L BA+0.4 mg/L IAA+3%蔗糖+6 g/L琼脂培养基上增殖生长良好。试管绿苗在1/4MS+0.5 mg/L IBA+2%蔗糖+6 g/L琼脂培养基上生根率达70%以上。

The growing shoots of jujube variety Luzao 1 were used to study the bud induction and rapid propagation of test tube plantlets.The results showed that the latent bud of shoot stem segments could be induced to break on the induction medium,which was MS supplemented with 1 mg/L BA,0.2 mg/L IBA and 3% sucrose,and the bud break rate was 54.8%.On the same induction medium,adventitious bud could be induced to regenerate from the distal cut surface of stem section not touch medium,and the regeneration rate was 23.8%.The propagation of adventitious bud and latent bud was better on the medium of MS＋2 mg/L BA＋0.4 mg/L IAA＋3%sucrose＋6 g/L agar.The rooting rate of test tube plantlets was over 70% on the medium of 1/4MS＋0.5 mg/L IBA＋2%sucrose＋6 g/L agar.