可同时测定蛇胆川贝液中贝母素甲与贝母素乙含量的方法

Simultaneous Determination of Peimine and Peiminine in Shedanchuan Solution by HPLC-ELSD

目的建立同时测定蛇胆川贝液中贝母素甲与贝母素乙含量的方法。方法采用Agilent SB C18色谱柱(150 mm×4.6 mm,5μm),以乙腈-水-二乙胺(70∶30∶0.03)为流动相,流速为1.0 mL/min,蒸发光散射检测器检测。结果贝母素甲与贝母素乙的进样量线性范围分别为0.060～2.406μg(r=0.999 7)和0.071～2.812μg(r=0.999 6),平均加样回收率分别为97.70%和97.90%,RSD分别为1.07%和1.34%(n=6)。结论所采用的方法便捷、有效,适用于检测贝母类生物碱等无紫外吸收的化学成分。

Objective To establish a method for simultaneously determining the contents of peimine and peiminine in Shedanchuanbei Solution.Methods The Agilent SB C18 coloumn（150 mm×4.6 mm,5 μm） was used with the evaporative light scattering detector（ELSD） for detection,acetonitrile-water-diethylamine（70 ∶30 ∶0.03） as the mobile phase and the flow rate of 1.0 mL/min.Results The linear ranges of peimine and peiminine were 0.060-2.406 μg（r=0.999 7） and 0.071-2.812 μg（r=0.999 6）,and the average recovery rates were 97.70%and 97.90%,respectively.Conclusion The method is convenient,effective and suitable for determining the chemical constituents of Fritillaria alkaloids without ultraviolet absorption.