谷氨酰胺对大鼠急性全脑缺血再灌注损伤后小肠黏膜sIgA表达的影响

Impact of Glutamine on the expression of sIgA of the intestinal mucosal barrier on the acute global cerebral ischemia-reperfusion injury in rats

目的通过建立大鼠全脑缺血再灌注模型,探讨急性全脑缺血再灌注损伤时应用谷氨酰胺后小肠黏膜sIgA表达的影响。方法采用四血管阻断法建立大鼠全脑缺血再灌注动物模型。实验分4组,分别为假手术组(除了不电凝椎动脉和夹闭双侧颈总动脉,其余与盐水对照组相同)、盐水对照组、奥扎格雷钠组和谷氨酰胺组。结果 sIgA表达水平:在6h、12h和24h,与假手术组相比,盐水对照组、奥扎格雷钠组和谷氨酰胺组均有不同程度表达下降,差异有统计学意义(P<0.05)。与谷氨酰胺组相比,盐水对照组和奥扎格雷钠组表达下降严重,差异有统计学意义(P<0.05),而盐水对照组和奥扎格雷钠组相比差异无统计学意义(P>0.05)。结论大鼠全脑缺血再灌注模型在急性期可造成小肠黏膜的sIgA表达水平下降,应用谷氨酰胺后可提高sIgA表达水平,从而在sIgA表达水平方面减轻大鼠全脑缺血再灌注对小肠黏膜的损伤。

Objective To study the impact of Glutamine on the expression of sIgA of the intestinal mucosal barrier on the acute global cerebral ischemia-reperfusion injury in rats through establishing the four-vessel occlusion rat model. Methods The four-vessel occlusion rat model was established, an resting isoelectric electroencephalogram （EEG） pattern which occurred fol- lowing bilateral carotid artery clamping was adopted as the standard for judging whether the rat got the global brain ischemic state. The brain of rats was reperfused after 10 minutes of the global brain ischemia. The rats were divided into four groups： the sham operation group （control group）, the saline group, the ozagrel group and the Glutamine group. Results At 6-hour, 12-hour and 24-hour, the expressions of sIgA in the saline group, ozagrel group and Glutamine group decreased in varying degrees（P〈0.05） ; the saline group and ozagrel group were the worst and there was no statistical significance （P〉0.05）. The expression of slgA in the Glutamine group was better than that in the saline group and ozagrel group, there was a significant difference（P〈0.05）. Conclusion The expression of slgA of the intestinal mucosal barrier decreased on the acute global cerebral ischemia reperfusion injury in rats. Glutamine can protect the intestinal mueosal barrier in the acute global cerebral ischemia-reperfusion injury in rats against the decrease of the expression of slgA.