吗啡处理对海马神经元细胞中钙调蛋白的影响

Influence of morphine on calmodulin in primary cultured hippocampal neurons

目的研究急、慢性吗啡处理对原代培养的海马神经元中钙调蛋白(CaM)的影响。方法取原代培养7d的成熟海马神经元,随机分为吗啡急性作用组(M1组)、纳洛酮急性戒断组(N1组)、吗啡慢性作用组(M2组)、纳洛酮慢性戒断组(N2组)及空白对照组(C组),每组6皿细胞。采用细胞免疫荧光染色(双抗体夹心法染色)技术及相对含量检测技术测定CaM的表达水平。结果荧光显微镜下各组都可见绿色荧光,表明在原代培养的海马神经元内存在CaM。M2组和N2组海马神经元内CaM的荧光强度明显高于C组(P<0.01)。结论慢性、多次使用成瘾药物才引起CaM表达增加,其机制可能是多次使用吗啡通过反复对细胞内信号转导途径的影响引起神经元细胞核CaM的改变。

Objective To investigate the influence of morphine on calmodulin in primary cultured hippocampal neurons. Methods Primary hippocampal neuronal cells of 7 days culture in vitro were randomly divided into 5 groups （n = 6）： acute morphine treatment, acute withdraw with naloxone, chronic morphine treatment, chronic withdraw with naloxone and control group. Control group was treated with saline. The CaM levels were detected with immunofluorescence technique. Results CaM levels increased significantly in both chronic morphine treatment and chronic naloxone withdraw treatment with more significance in withdraw group, while it kept unchanged in acute morphine treatment and acute naloxone withdraw group. Conclusion Chronic morphine treatment can increase the level of CaM. The mechanism may be related to intracellular signal transduetion.