大鼠后足切割后脊髓和背根神经节中脑源性神经营养因子表达的变化

The changes of BDNF in the spinal cord and dorsal root ganglion following hindpaw-incision in the rats

目的观察大鼠后足切割后脊髓和背根神经节(DRG)中脑源性神经营养因子(BDNF)水平的变化及其定位。方法雄性SD大鼠24只,体重150～250g,均施行后足切割手术。术后1h、6h、1d、3d各取6只大鼠行行为学检测后处死,分离脊髓和DRG进行免疫组化和双标免疫荧光检测BDNF。结果大鼠后足切割后,切割侧腰段脊髓背角和DRG中BDNF水平上升(P<0.05)。而对侧腰段和胸段脊髓中BDNF水平均没有明显改变。升高的BDNF主要定位于神经元而非星形胶质细胞或小胶质细胞。结论大鼠后足切割后切割侧腰段脊髓背角和DRG中BDNF表达上升,BDNF升高主要定位于神经元。

Objective To evaluate the level and location of brain derived neurotrophic factor （BDNF） in the spinal cord and dorsal root ganglion （DRG） following hindpaw-incision in rats. Methods Twenty-four male SD rats weighing 150-250 g, received hindpaw incision surgery. Paw withdraw threshold to mechanical stimuli was measured 1 hour, 6 hours, 1 day, 3 days after the operation with 6 rats in each time point. BDNF in spinal cord and dorsal root ganglion were analyzed later. Results After hind-paw incision, the level of BDNF was up-regulated in the lateral area of lumbar spinal cord and DRG （P〈0. 05） with their location in the neurons whereas thoracic BDNF remained unchanged. Conclusion BDNF in the spinal cord and DRG can be up-regulated after hindpaw incision in the rats. The increased level of BDNF was mainly localized in the neurons.