青海不同生态环境下蚕豆蛋白亚基差异性研究

A Study on the Difference of Protein-subunit in Faba Bean under Different Eco-types in Qinghai

以青海不同生态环境条件下的青海13号蚕豆为材料,利用SDS-PAGE电泳技术分离清蛋白和球蛋白各主要亚基,通过Band Scan 5.0软件得出各亚基的相对含量。结果表明,蚕豆清蛋白和球蛋白亚基构成具有差异性,发现2个99 ku、61 ku的清蛋白特异亚基和3个94 ku、64 ku、39 ku的球蛋白特异亚基,57 ku、47 ku等2个清蛋白亚基和61 ku、51 ku等2个球蛋白亚基具有一定的缺失现象。各亚基在含量上也各不相同,存在一定差别,可以看出,蚕豆蛋白亚基的增失及含量随生态环境的不同存在一定的变异。本研究对蚕豆品质育种具有一定的参考价值。

In order to investigate the effects of different ecological environments in Qinghai on the protein subunits in faba bean,the variety of Qinghai 13 under different eco-types in Qinghai Province was analyzed by SDS polyacrylamide gel electrophoresis（SDS-PAGE）.Based on the analysis above,various main subunits of albumin and globulin were separated and their relative contents were acquired by Band Scan5.0.The result showed that there were differences in the formation of the albumin and globulin subunits in faba bean.Albumin contained 20 subunits of 18 bases and 2 specials,globulin contained 13 subunits of 10 bases and 3 specials.The special subunits were 99 ku,61 ku a of albumin and 94 ku,64 ku,39 ku of globulin.The lacking subunits were 57 ku,47 ku of albumin and 61 ku,51 ku of globulin.The quantities of subunits were different from each other because of the different ecological environments.