绿草定-2-丁氧基乙酯对竹林土壤微生物的影响

The Impact of Triclopyr-2-butoxyethylester on Microbe in Bamboo Forest Soil

采用室内培养的方法,研究了绿草定-2-丁氧基乙酯在不同施药剂量下对竹林土壤微生物种群和生物量碳的影响。土壤微生物种群的影响研究采用平板培养法,土壤微生物生物量碳的影响研究采用熏蒸-提取法,提取液中的有机碳采用总有机碳分析仪测定。结果表明,绿草定-2-丁氧基乙酯对细菌高浓度时有抑制作用,低浓度时有刺激作用,生产用量(1.8 mg/kg)在培养的25 d内可恢复到对照水平。真菌培养结果表明,各浓度均有刺激作用,且随着浓度的增大刺激作用增强,生产用量在培养的40 d内可恢复到对照水平。放线菌培养结果表明,较低浓度时有刺激作用,较高浓度时有抑制作用,生产用量在培养的25 d内可恢复到对照水平。绿草定-2-丁氧基乙酯低浓度时土壤微生物生物量碳增加,高浓度时土壤微生物生物量碳减小,生产用量在40 d内恢复到对照水平。随着培养时间的延长,各浓度对土壤微生物的影响均减小,可能与绿草定-2-丁氧基乙酯的缓慢降解有关。模拟非均匀施药情况的10倍生产用量(18 mg/kg),60 d内可恢复到对照水平,说明绿草定在合理用药的情况下对土壤微生物影响较小,不会构成长期不可恢复的影响。

The effects of triclopyr-2-butoxyethylester under different concentrations on bamboo forest soil microbial populations and biomass carbon were studied through laboratory incubation experiment.The study on soil microbial population used plate culture method.The study on microbial biomass carbon used fumigation-extraction method,and the organic carbon in the extract was quantified with organic carbon analyzer.The results indicated that high concentrations of triclopyr-2-butoxyethylester had an inhibiting effect and low concentrations had a stimulating effect.The production amount（1.8 mg/kg） could recover to the control level within 25 days.Fungal culture results showed that all the concentrations had stimulating effect and it increased with concentration increasing.The production amount could recover to the control level within 40 days.Actinomycetes culture results showed that lower concentrations had a stimulating effect,and higher concentrations had a inhibiting effect.The product amount could recover to the control level within 25 days.Soil microbial biomass increased in low concentrations of triclopyr-2-butoxyethylester,and reduced in high concentration.The production amount could recover to the control level within 40 days.With the culture time,the effect of all concentrations on soil microbes reduced,which might be related to the slow degradation of triclopyr-2-butoxyethylester.10 times production amount（18 mg / kg） simulating non-uniform spraying condition could recover to the control level within 60 days,which indicated that triclopyr-2-butoxyethylester in rational use amount could have less impact on bamboo forest soil microbial populations,and would not constitute a long-term non-recovery effect.