油茶籽油水酶法制取机制的显微研究

A Study on the Mechanism of Aqueous Enzymatic Extraction of Camellia oleifera Seed Oil

使用透射和扫描电子显微镜观察经水酶法提油前后的油茶籽仁细胞内部及表面形态结构的变化。实验结果表明:油茶籽仁细胞内油脂体呈离散状分布;Alcalase 2.4 L碱性蛋白酶能有效降解油茶籽仁细胞的细胞壁并释放包括油脂在内的细胞内容物,其作用过程是一个动态的降解过程。

By means of TEM and SEM the intracellular and extracellular changes in Camellia oleifera seed cells during aqueous enzymatic extraction of oil from it were observed.The result showed that the lipid granules were discretely distributed in camellia seed cells,and it was confirmed that Alcalase 2.4 L could degrade cell wall effectively and release cell contents including lipid granules from the morphological view.The degradation of cell wall was a dynamic process.