摘要
运用SRAP技术对哲罗鲑、细鳞鲑及杂交种(哲罗鲑♀×细鳞鲑♂)的遗传结构进行分析。结果表明:(1)哲罗鲑群体和细鳞鲑群体迁移率相同的位点分别占所扩增条带的48.29%和48.53%,表明二者在遗传结构上具有较大的相似性。杂交群体所扩增的位点中,具有哲罗鲑群体特异位点为30.30%,具有细鳞鲑群体特异位点为25.54%,具有2群体共有位点为39.39%,属两性融合生殖。(2)3个群体多样性水平从高到低排列为细鳞鲑、杂交种、哲罗鲑,并且杂交种表达出一定的杂种优势;(3)检测到种间特异位点53个,将这些特异位点部分组合,可以有效进行杂种鉴定;(4)杂交种与哲罗鲑和细鳞鲑的遗传距离分别为0.255 3和0.385 8,表明杂交种与两亲本群体的遗传差异是不对等的,偏向母本一方。
The genetic structure of three populations(Hucho taimen and Brachymystax lenok and hybrids) was analyzed by SRAP molecular marker.The results are as follows:(1) Hucho taimen and Brachymystax lenok have percentages of 48.29% and 48.53% of all bands,the result showed very high genetic identity in the two populations.In the hybrids,the amplified fragments include 30.30% female parent-specific bands,25.54% male parent-specific bands and 39.39% mutual bands,the hybridization between the parents are gamogenesis and most genetic material come from both of the parents.(2)The abundance of genetic diversity in the three populations arranged in a decent order is Brachymystax lenokhybridsHucho taimen,and the hybrids have obvious heterosis.(3)53 interspecies specific loci were screened out.Combining part of those loci can effectively identify the hybrids.(4) The hybrids have a genetic distance of 0.255 3 and 0.385 8 from Hucho taimen and Brachymystax lenok,separately,which elucidates the hybrids have an unequal heredity difference to their parents and a genetic inclination to their female parent.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第6期1187-1194,共8页
Acta Agriculturae Universitatis Jiangxiensis
基金
公益性行业科研专项(201003055)
黑龙江水产研究所基本科研专项资金资助(2008HSYZX-SJ-08)
关键词
哲罗鲑
细鳞鲑
杂交种
遗传分析
Hucho taimen
Brachymystax lenok
hybrid
Genetic analysis