青橄榄叶提取物体外抑菌活性研究

Antimicrobial activity of different polar extracts from the leaves of Chinese olive in vitro

【目的】测定青橄榄叶提取物及大孔树脂AB-8不同极性洗脱产物对供试菌株的体外抑菌活性。【方法】采用96孔板微量稀释法测定青橄榄叶总提取物及不同极性洗脱产物对白色念珠菌、铜绿假单胞杆菌、耐甲氧西林金黄色葡萄球菌、耐药白色念珠菌、酿酒酵母和金黄色葡萄球菌的最低抑菌浓度(MIC)。【结果】大孔树脂水洗极性部位对白色念珠菌的MIC为20.62 g/ml,50%乙醇洗脱部分对铜绿假单胞杆菌的MIC为82.5μg/ml,90%乙醇洗脱部分对白色念珠菌、铜绿假单胞菌、耐甲氧西林金黄色葡萄球菌、耐药白色念珠菌、酿酒酵母和金黄色葡萄球菌的MIC分别为41.25,20.62,20.62,10.32,165.0和2.58μg/ml。【结论】青橄榄叶洗脱产物的水、50%乙醇及90%乙醇洗脱部分对白色念珠菌、铜绿假单胞杆菌、耐甲氧西林金黄色葡萄球菌、耐药白色念珠菌、酿酒酵母和金黄色葡萄球菌有较好的抑菌活性,尤其是90%乙醇洗脱部位的抑菌范围广,活性强且呈现量效依赖关系。

【Objective】 To study the antibacterial activities of extract from the leaves of Chinese olive and different pole extraction chromatographied on macroporous resin AB-8 in vitro.【Methods】 The Minmum Inhibiting Concentration（MIC） of extracts of Chinese olive leaves and different polar extracts chromatographied on macroporous resin AB-8 to Candida albicans,Pseudomonas aeruginosa,MRSA,resistant Candida albicans,Saccharomyces cerevisiae and Staphylococcus aureus were measured by microdilution method on 96-well plates.【Results】 The water-eluted part of extract of leaves of Chinese olive isolated by macroporous resin AB-8 showed notable inhibiting microorganism activity to Candida albican and the MIC was 20.62 μg/ml.The 50% ethanol-eluted part had a better inhibiting microorganism activity to Pseudomonas aeruginosa and the MIC was 82.5 μg/ml.The 90% ethanol-eluted part showed a broadspectrum antibacterial activies： the MIC of Candida albican、Pseudomonas aeruginosa、MRSA、resistant Candida albicans、Sacharomyces cervisiae and Staphylococcus aureus respectively was 41.25、20.62、20.62、10.32、165.0 and 2.58 μg/ml.【Conclusion】 The different polar extracts of leaves of Chinese olive exhibits potential antimicrobial activities.Especially,the 90% ethanol-eluted part shows a broadspectrum activies against Candida albicans,Pseudomonas aeruginosa,MRSA,resistant Candida albicans,Saccharomyces cerevisiae,Staphylococcus aureus and exhibited better dose-effect relationship.