战备消毒包内细菌来源分析

Experimental study on the source bacteria in sterile packages for war readiness

【目的】探讨高原地区自然环境中战备消毒包内细菌来源。【方法】根据战备消毒包内金黄色葡萄球菌、木糖葡萄球菌、蜡样芽胞杆菌和人葡萄球菌筛选战备仓库空气同种细菌不同菌株,提取基因组DNA,以其为模板,对16SrRNA和23SrRNA的转录间隔区(internal transcribled spacer,ITS)序列进行PCR扩增,将扩增产物回收测序,结果用Blast进行比对。【结果】金黄色葡萄球菌、木糖葡萄球菌、蜡样芽胞杆菌和人葡萄球菌DNA PCR扩增产物分别在1000 bp、1100 bp、850 bp、1000 bp左右显现单一特异性条带;ITS序列相似性约为99%～100%。【结论】战备消毒包内细菌来源于空气细菌。

【Objective】 To investigate the source of bacteria in sterile packages for war readiness under natural environment in highland region.【Methods】 Genomic DNAs of Staphylococcus aureus,Staphylococcus xylose,Bacillus cereus and Staphylococcus hominis from sterile packages for war readiness and air bacteria strains in war readiness warehouse were extracted,and then used as templates to amplify the 16SrRNA and 23SrRNA internal transcribed spacer（ITS） sequences by PCR.PCR products were extracted and sequenced,and the sequences homology analysis was performed with BLAST.【Results】 Amplification products of DNA PCR from Staphylococcus aureus,Staphylococcus xylose,Bacillus cereus and Staphylococcus hominis were about 1000 bp,1100 bp,850 bp and1000 bp respectively;The 16SrRNA-23SrRNA ITS sequences of bacteria strains in sterile packages shared 99 % to 100 % homology with those from the air bacteria strains in war readiness warehouse.【Conclusion】 The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment.