芡实壳中脂肪酸及其微量元素的测定

Study on fatty acid and trace elements of Euryale Ferox shell

分析了芡实壳中微量元素和脂肪酸的含量。用石油醚作溶剂在索氏提取器中提取芡实壳的脂肪酸,0.5mol/L氢氧化钠-甲醇溶液做甲酯化处理,气相色谱-质谱联用法进行定性分析,按峰面积归一化法,求出脂肪酸成分的百分含量。经硝酸-高氯酸体系消解,应用电感耦合等离子体原子发射光谱法测定芡实壳微量元素的含量。结果表明,从脂肪酸中鉴定出8个化学组分,和占总成分的83.72%,主要成分为棕榈酸、亚油酸和油酸,含量分别为10.72%、28.35%、12.62%;检测出14种微量元素,其中铜、锰、锌和铁的含量较高,分别为0.248 mg/g、0.164 mg/g、0.217 mg/g和0.305 mg/g。

The contents of trace elements and fatty acids in Euryale Ferox shell were analysed.The fatty acids in the Euryale shell were extracted with petroleum ether in Soxhlets extractor,using 0.5mol/L NaOH-CH3OH as the methyl esterification solvent and separated by GC-MS.The constituents of fatty acids were identified and their amount were determined by normalization method.Sample of Euryale shell was digested with a mixture of HNO3 and HClO4,and contents of trace elements were determined by ICP-AES.The result shows that 8 compounds were identified,accounting for 83.72% of its total content.The main components were palmitic acid,linoleic acid and oleic acid,their contents were 10.72%,28.35% and 12.62%.Fourteen kinds of trace elements were obtained,Euryale shell was rich in Cu,Mn,Zn and Fe with the contents of 0.248 mg/g,0.164 mg/g,0.217 mg/g and 0.305 mg/g.