大黄素对人肾成纤维细胞增殖的影响

Effect of Emodin on Human Kidney Fibroblast Proliferation

目的:研究大黄素对人肾成纤维细胞增殖的影响。方法:在体外人肾成纤维细胞培养的基础上,采用3HTdR掺入法观察了不同浓度大黄素(10、30、50、80、100μg/ml)对人肾成纤维细胞3HTdR掺入率的影响。同时,利用流式细胞仪观察了大黄素(10、50、100μg/ml)对细胞周期的影响。结果:大黄素以剂量依赖方式降低了人肾成纤维细胞3HTdR掺入率(r=0995,P<001)。大黄素(50和100μg/ml)抑制了人肾成纤维细胞细胞周期的进程(P<001)。结论:大黄素通过抑制细胞DNA合成,延迟细胞周期的进程,抑制了细胞增殖,这一结果为大黄素的临床应用提供了部分实验依据。

To study the effect of emodin on proliferation of human kidney fibroblasts in vitro. Methods: After human kidney fibroblasts were cultured, isolated and identified, both the effects of five different concentrations of emodin (10, 30, 50, 80 and 100μg/ml) on 3 H TdR incorporation, and the effects of three different concentratioins of emodin (10, 50 and 100μg/ml) on cell cycle by flow cytometry were investigated. Results: The exposure of human kidney fibroblasts to emodin (10～100μg/ml) caused a dose dependent reduction in 3 H TdR (r=0 995,P<0 01), and could delay the progress of human kidney fibroblasts from G 1 to S phase. Conclusions: Emodin inhibited the proliferation of human kidney′s fibroblasts by inhibiting the cell DNA synthase and delaying the progress of cell cycle. These findings might provide part of experimental basis for the clinical use of emodin