三-(2,3-二溴丙基)异氰脲酸酯和17β-雌二醇复合暴露对雄性斑马鱼的毒性效应

Toxic effects on adult male zebrafish(Danio rerio) following co-exposure to tris-(2,3-dibromopropyl) isocyanurate and 17 β-estradiol

三-(2,3-二溴丙基)异氰脲酸酯(TBC)是近年来在环境中新检测到的一种杂环溴化阻燃剂,但其毒性效应尚不明确.我们以雄性斑马鱼为受试动物,开展了TBC和17β-雌二醇(E2)的复合暴露实验,以研究TBC潜在的致毒机制.结果显示TBC可显著抑制E2所引起的斑马鱼肥满度指数上升,并能改善E2暴露所造成的斑马鱼精子细胞比例降低和性腺发育迟缓;E2暴露可造成斑马鱼肝组织脂肪样病变,肝中MDA含量上升,但SOD活力没有显著改变;TBC复合暴露后,MDA含量呈现下降趋势,但鱼肝仍有明显的脂肪样病变,对肝脏中的SOD活力也没有显著影响;更重要的是,TBC可显著抑制E2单一暴露所诱导的雄鱼肝脏中卵黄蛋白原基因的表达,这些数据表明TBC具有明显的内分泌干扰效应.因此,需要进一步关注TBC这类杂环溴化阻燃剂对水环境的潜在危害.

Tris-（2,3-dibromopropyl） isocyanurate（TBC） is a heterocyclic brominated flame retardant that was recently detected in the environment,and its toxic effects are still unclear.In this paper,adult male zebrafish were exposed to TBC and 17β-estradiol（E2） to evaluate the potential toxic mechanism of TBC.We found that TBC could significantly inhibit the raise of condition factor and could also alleviate the low proportion of spermatids and the development retardant of the sex gland,which were induced by E2.The exposure of E2 led to fatty degeneration of the hepatocytes and increased MDA content,but SOD activity in zebrafish liver remained unchanged.On the other hand,the co-exposure of TBC and E2 could alleviate the MDA enhancement,while the hepatocytic fatty degeneration and the SOD activity remained unchanged.Moreover,TBC could significantly suppress the increase expression of vitellogenin gene in liver which was induced by E2.These results suggested that TBC could cause obvious endocrine disruption in zebrafish.Therefore,there is a need for further characterizing the toxicity of heterocyclic brominated flame retardant,such as TBC,as they may cause potential contamination to aquatic ecosystems.