摘要
建立了一种高效阴离子交换-脉冲安培检测(HPAE-PAD)测定化妆品中海藻糖的方法。采用不同的样品前处理方法,用METROSEP CARB 1分离柱(150 mm×4.0 mm)进行分离,以200.0 mmol·L-1的NaOH溶液为流动相进行洗脱,7 min内可完成对样品的分析。结果表明,海藻糖的检出限为0.02 mg·L-1,峰面积的相对标准偏差为0.17%,保留时间的相对标准偏差为0.28%;化妆品中常见的甘油和丙二醇不会对其测定产生干扰,平均回收率为95.2%~99.2%。
An analysis method for determining trehalose in cosmetics based on high performance anion exchange chromatography with pulsed amperage detection ( HPAE - PAD) was established. Various sample pretreatment methods were adopted. The samples were analyzed by ion chromatography with METROSEP CARB 1 column (150 mm 4. 0 mm). NaOH solution of 200. 0 mmol · L-1 was used asthe mobile phase for elution. Analysis of a sample can be completed within 7 min. The detection limit of trehalose was 0. 02 mg · L-l. The RSD of peak area achieves 0. 17%, and the RSD of retention time achieves 0. 28%. Glycerol and propylene glycol which can be easily found in cosmetics have no interference on the determination of the trehalose. The average recoveries achieves 95.2% - 99. 2%.
出处
《日用化学工业》
CAS
CSCD
北大核心
2012年第1期76-78,共3页
China Surfactant Detergent & Cosmetics
基金
科技部重大专项资助项目(2008ZX07315)
重庆市自然科学基金资助项目(CSTC2007BB5370)
关键词
化妆品
海藻糖
离子色谱法
脉冲安培检测
cosmetics
trehalose
ion chromatography
pulsed amperage detection