离子色谱法测定化妆品中的海藻糖

Quick determination of trehalose in cosmetics by ion chromatography

建立了一种高效阴离子交换-脉冲安培检测(HPAE-PAD)测定化妆品中海藻糖的方法。采用不同的样品前处理方法,用METROSEP CARB 1分离柱(150 mm×4.0 mm)进行分离,以200.0 mmol·L-1的NaOH溶液为流动相进行洗脱,7 min内可完成对样品的分析。结果表明,海藻糖的检出限为0.02 mg·L-1,峰面积的相对标准偏差为0.17%,保留时间的相对标准偏差为0.28%;化妆品中常见的甘油和丙二醇不会对其测定产生干扰,平均回收率为95.2%～99.2%。

An analysis method for determining trehalose in cosmetics based on high performance anion exchange chromatography with pulsed amperage detection （ HPAE - PAD） was established. Various sample pretreatment methods were adopted. The samples were analyzed by ion chromatography with METROSEP CARB 1 column （150 mm 4. 0 mm）. NaOH solution of 200. 0 mmol · L-1 was used asthe mobile phase for elution. Analysis of a sample can be completed within 7 min. The detection limit of trehalose was 0. 02 mg · L-l. The RSD of peak area achieves 0. 17%, and the RSD of retention time achieves 0. 28%. Glycerol and propylene glycol which can be easily found in cosmetics have no interference on the determination of the trehalose. The average recoveries achieves 95.2% - 99. 2%.