Effects of electroacupuncture at "Nèiguān" (内关 PC6) on the catecholamine neurotransmitters of rats with myocardial hypertrophy

电针“内关”穴对心肌肥厚大鼠儿茶酚胺类神经递质的影响(英文)

Objective To study the neuroendocrine mechanisms of electroacupuncture （EA） at ＂Neiguan＂ （内关 PC 6） for preventing and treating myocardial hypertrophy. Methods Thirty 3-month-old Sprague-Dawley rats were randomly divided into a normal group, a model group and an EA group according to the random digit table, 10 in each group. Isoprenaline Hydrochloride （ISO） injection was subcutaneously injected to establish the model. EA at ＂Neiguan＂ （内关 PC 6） was applied for rats in the EA group, 20 min each time, once daily, for 14 successive days. No treatment was given to rats in the normal group and the observed. The plasma catecholamine neurotransmitters levels and c determined with high performance liquid chromatography model group. The electrocardiogram （ECG） was hanges of their metabolic products contents were （HLPC） method. Results Compared with the normal group, catecholamine neurotransmitters, such as epinephrin （E）, norepinephrine （NE）, and dopamine （DA） and metabolic products, such as 3-methoxy-4-hydroxyphenylglycol （MHPG）, homovanillic acid （HVA）, and 3,4-dihydroxy-phenyl acetic acid （DOPAC） were significantly increased in the model group （all P〈0.05）. These parameters decreased in the EA group when compared with the model group （all P〈0.05）. Conclusion EA at ＂Neiguan＂ （内关 PC 6） could improve the myocardial hypertrophy, which might be correlated with the neuroendocrine system.

目的:探讨针刺"内关"穴防治心肌肥厚的神经内分泌机制。方法:3月龄SD大鼠30只,按随机数字表法分为3组,正常组、模型组、电针组,每组10只,造模采用皮下注射盐酸异丙肾上腺素(Isoprenaline Hydrochloride,ISO)注射液,电针组采用电针"内关"穴治疗,每日1次,每次20 min,连续14 d。正常组与模型组不予治疗。观察心电图,高效液相色谱法(high performance liquid chromatography,HLPC)测定血浆中儿茶酚胺神经递质及其代谢产物的含量变化。结果:与正常组比较,模型组大鼠血浆中儿茶酚胺类神经递质肾上腺素(E)、去甲肾上腺素(NE)、多巴胺(DA)及代谢产物3-甲氧基-4-羟基苯乙二醇(MHPG)、高香草酸(HVA)、3,4-二羟基苯乙酸(DOPAC)的含量明显升高,差异具有统计学意义(均P<0.05);与模型组相比,电针组大鼠血浆中儿茶酚胺类神经递质及代谢产物含量减少,差异具有统计学意义(均P<0.05)。结论:针刺"内关"穴可以改善心肌肥厚,这与通过调节神经内分泌的作用有关。