肌营养不良蛋白聚糖在胃癌原发灶与转移灶中的表达及临床意义

Expression of α-dystroglycan in primary and metastatic lesions of gastric cancer and its clinical significance

目的探讨肌营养不良蛋白聚糖（DG）在胃癌发生、发展中的表达变化及其与临床病理特征的关系。方法选择20例经手术证实的合并淋巴结及远处转移且病理资料完整的胃癌患者，分别对患者的正常胃黏膜、胃癌原发灶、淋巴结转移灶以及远处转移灶的石蜡标本采用免疫组织化学染色（SP法）检测DG亚基（α—DG）的表达，并分析其与相关临床和病理特征之间的关系。结果α—DG在正常黏膜、胃癌原发灶、转移淋巴结及远处转移灶的阳性表达率分别为95％、70％、25％、5％；原发灶中α—DG的阳性表达率显著低于正常黏膜，且高于淋巴结转移灶及远处转移灶（P〈0．01）；OC—DG在印戒细胞癌中的阳性表达率低于乳头状腺癌、管状腺癌和低分化腺癌（P〈0．05）；而OC—DG在不同大体病理分型、肿瘤分化程度、浸润深度及淋巴结分期者之间阳性表达率的差异则均无统计学意义（均P〉0．05）。结论检测DG在胃癌组织中的表达水平有助于了解其生物学行为，且DG在胃癌的发生和发展中可能起重要作用。

Objective To investigate the expression of α-dystroglycan （α-DG） in primary and metastatic lesions of gastric carcinoma and its clinical significance. Methods Expression of α-DG was detected by immunohistochemistry in parafn-embedded surgical specimens of normal gastric mucosa, primary loci, metastatic lymph node foci and distant metastatic foci from 20 patients with gastric adenocarcinoma. Correlations between α-DG expression and clinicopathological features of gastric cancer were retrospectively analyzed. Results The expressions of α-DG were 95%, 70%, 25% and 5% in normal gastric mucosa, primary foci, lymph node metastatic loci and distant metastatic foci, respectively（P〈0.05）. The expression of α -DG was lower in signet cell cancer than that in papillary adenocarcinoma, tubular adenocarcinoma and poorly differential adenocarcinoma （P〈0.05）. But the gross type, histological differential grade, lymph node stage and invasive depth showed no significant correlation with the expression level of α-DG in the primary tumor. Conclusion DG may be involved in the tumorgenesis and progression of gastric carcinoma. The detection of expression level of α-DG may be of value to predict the malignant behavior of the tumor.