摘要
目的 通过对去卵巢小鼠补充E2治疗,观察外周血T淋巴细胞亚群及免疫球蛋白水平变化,探讨E2对上述指标的影响.方法 60只健康雌性小鼠随机分为3组,假手术组不给药物,去卵巢小鼠分别接受苯甲酸雌二醇（用药组）及注射油（非用药组）皮下注射治疗,每周2次,共注射25次（12周）,酶联免疫吸附法检测3组血清E2及免疫球蛋白（IgG、IgM、IgA）水平,流式细胞仪检测血CD4＋ T细胞和CD8＋T细胞变化.结果 （1）术后12 周用药组血CD8＋T细胞数（18.16±4.04）比非用药组（25.73±3.63）降低（P＜0.05）;假手术组血CD8＋T细胞数（16.54±3.12）比非用药组（25.73±3.63）降低（P＜0.05）,差异有统计学意义.（2）与非用药组比较,用药组及假手术组CD4＋ T细胞数呈上升趋势,但差异均无统计学意义（均P ＞0.05）.（3）血清IgG、IgM、IgA水平3组比较差异均无统计学意义（均P ＞0.05）.结论 E2可降低去卵巢小鼠外周血CD8＋T细胞水平,参与免疫系统调节.
Objective To investigate the relationship between estradiol （ E2）and the levels of peripheral T cell subsets and immunoglobulins in ovariectomized mice. Methods Sixty female mice were divided into 3 group at random： sham operation group, OVX＋EB group and OVX group. In OVX＋EB group ovariectomized mice were injected with estradiol twice a week for 25 times, in OVX group ovariectomized mice were treated with injection oil instead. The serum levels of E2 and immunoglobulin G, M, A were measured by ELISA; peripheral blood CD4＋ and CD8＋levels were measured by flow cytometer. Results The CD8＋ levels in OVX＋EB group and sham group were significantly lower than those in OVX group （ 18.16 ± 4.04 and 16.54 ± 3.12vs 25.73 ± 3.63, both P〈0.05）. There were no differences in CD4＋ levels and immunoglobulin （G,M,A）levels among three group. Conclusion E2 can decrease the peripheral blood CD8＋levels in ovariectomized mice.
出处
《浙江医学》
CAS
2011年第12期1793-1795,共3页
Zhejiang Medical Journal
基金
金华市科技局项目（J2008-3-030）
