日粮粗蛋白质水平对鹅组织IGF-I mRNA表达的影响

Effects of dietary crude protein levels on IGF-I mRNA expression in some tissues of geese

为了探明不同粗蛋白质(CP)水平日粮对鹅组织中胰岛素样生长因子-I基因(IGF-I)mRNA表达量的影响,为科学配制肉鹅饲料提供理论依据,采用半定量RT-PCR方法检测分别饲喂粗蛋白质水平为16%、18%、20%日粮的28日龄苏牧白鹅的肝脏、腿肌和胸肌组织的胰IGF-I mRNA表达量差异。结果表明:在不同粗蛋白质水平下鹅的肝脏和胸肌组织中均能检测到IGF-I mRNA表达,而在腿肌中未能检测到该基因mRNA表达。在肝脏组织中,18%CP组IGF-I mRNA表达量低于16%CP组和20%CP组,后两者之间无显著差异。在母鹅胸肌组织中,各组IGF-I mRNA表达量无显著差异;但20%CP组公鹅的IGF-I mRNA表达量显著高于16%CP组。20%CP组公鹅IGF-I mRNA表达量显著高于母鹅,18%CP组、20%CP组公鹅肝脏组织IGF-I mRNA表达量极显著低于母鹅,其他组公母间差异均不显著。不同粗蛋白质水平能影响鹅组织中IGF-I mRNA表达;在相同粗蛋白质水平的日粮和同性别条件下,IGF-I mRNA相对表达量肝脏大于胸肌,腿肌中未检出。

This experiment was conducted to study the effect of dietary crude protein（CP） levels on insulin-like growth factor gene IGF-I mRNA expression level in some tissues of 4-week-old Sumu white geese using semi-quantitative RT-PCR,to provide a theoretical basis for scientific preparation of geese feed.The geese were fed to 28 days old,and dietary CP levels were 16%,18% and 20%.The results showed that the IGF-I mRNA could express in liver and chest muscle,but not in leg muscle.The IGF-I mRNA expression level of 18% CP group was lower than those of 16% CP and 20% CP groups,and the difference between the latter two groups was not significant（P0.05）.The IGF-I mRNA expression level did not differ（P0.05） in the chest muscle of females among different groups,but for males,the expression level of 20% CP group was significantly higher than that of 16% CP group（P0.05）.The IGF-I mRNA expression level of males was higher than females in 20% CP group,and that in liver of males was significantly lower than females in 18% CP group and 20% CP group.The IGF-I mRNA expression level in other groups had no significant difference between sexes.Different dietary CP levels could affect the IGF-I mRNA expression levels.Under the same conditions of CP level and sex,the IGF-I mRNA expression level in liver was higher than that in chest muscle,but there was no expression in leg muscle.