白腐菌Polyporus varius的木质素降解及其对芦苇综纤维素含量和kappa值的影响

Lignin Degradation by the White Fungus Polyporus varius and its Effect of Pretreatment Conditions on Holocellulose Content and Kappa Number in Phragmites Australis

用苯胺蓝法结合鞣酸法从枯树木片中筛选了一株具有降解木质素能力的白腐菌菌株NM23-002,初步鉴定为多孔菌属Polyporus varius,并研究了其对芦苇综纤维素含量的影响及降低纸浆中kappa值的条件.结果表明,P.varius预处理造纸原料芦苇的最佳工艺条件为pH 5.5,温度41℃,接种量为50 v/v,处理时间16 d,此时kappa值最低为17.851,与对照样相比kappa值变化非常明显,降低了2.236,而芦苇综纤维素被降解不明显,被降解了0.003%.同时,木质素的相对降解率在20 d后达到37.44%,脱木素选择性为8.83,表明能起到良好的降解木质素的作用.

In this study,Aniline Blue Staining Method（ABSM） together with Tannic Acid Method（TAM） is utilized to screen the stain which is able to degrade lignin from the film of dead trees.The fungus is identified as Polyporus varius and its effects of pretreatment conditions on holocellulose content and kappa number in reed paper-pulping are studied.The results indicate that the optimum pretreatment conditions of Papermulberry by P.varius are shown as follows： pH 5.0,temperature 41℃,inoculum 50v/v,processing time 16d.At this point,the minimum kappa number is 17.851,with the reducing number of 2.236 compared with the control sample.Kappa number changes obviously,and the degradation of Phragmites australis holocellulose is not obvious,with the reducing degradation of 0.003%.Also,the degradation rate of lignin is investigated,and the degradation rate and delignification selectivity are 37.44% and 8.83,respectively.It indicates that the selected fungus has the ability to decompose lignin effectively and selectively.