敌敌畏农药的极性暴露对泥鳅的遗传毒性及生理影响

Genetics Toxicity and Physiology Toxicity of Dichlorovos to Misgurnus Anguillicaudatus

以泥鳅为受试动物,研究敌敌畏农药稀释液对泥鳅的急性毒性、遗传毒性及生理的影响.急性试验表明:敌敌畏稀释液对泥鳅具有一定的毒性,其对泥鳅96 h的LD50为6.409μg.L-1.微核试验表明:对照组红细胞微核率为0.195‰,而处理组红细胞微核率最高达到2.138‰,泥鳅红细胞微核率各处理组均明显高于对照组(P<0.01).生理试验表明:处理组肝组织的GPT和GOT活力均明显低于对照组,且随农药稀释液浓度增高而减小.由上可知,敌敌畏农药稀释液对泥鳅有显著的遗传毒性及生理影响.

To investigate the pesticide residues effects of the genetics toxicity and physiology toxicity on Misgurnus anguillicaudatus,toxicological testing has been perfomed by Dichlorovos.The results show that the diluted Dichlorovos are toxic to Misgurnus anguillicaudatus and its LD50 of 96 h is 6.409 μg·L-1.Using the micronucleus（MN） technique,the genotoxicity of Dichlorovos is measured on the Misgurnus anguillicaudatus＇s peripheral blood cells.The results indicated that the highest the frequencies of MN is 2.138‰ in test group,while the frequencies of MN just only is 0.195‰ in control group.In other words,the Dichlorovos is likely to induce significant increases in the frequencies of MN,and there is significant difference in comparison with the controlled group.To analyse the physiological toxicity,the activity changes of glutamic-oxalacetic transaminase（GOT） and alanine aminotransferase（GPT） in liver are measured using xanthine oxidase enzyme assay.The results show that the activities of GOT and GPT in liver of the test group are lower than those of the control group（P0.05）,and it is discovered that when the residue concentration increase,the activities of GOT and GPT decrease.To sum up,the Dichlorovos residues are likely to induce significant genetics toxicity and physiological toxicity on loach.