紫外线照射对P53基因表达的影响

The effect of UV light radiation on tumor suppressor gene p53 expression

目的 探讨紫外线照射对抑癌基因P5 3表达的影响。方法 以P5 3基因功能正常的舌癌细胞株为靶细胞 ,用不同剂量的紫外线照射或相同剂量紫外线照射不同时间后 ,采用免疫沉淀法检测P5 3基因表达水平的变化。结果 紫外线照射可诱导P5 3基因过度表达。P5 3基因的过度表达在紫外线照射后可持续相当长一段时间。结论 外源性DNA损伤因子可诱导P5 3基因表达 ;P5

Objective The goal of this study was to analyze the effect of UV light radiation on p53 gene expression.Methods Tongue cancer cell line Tca83 was used to evaluate the relationship between UV light radiation and p53 gene expression. After different or same dosage of UV irradiation, p53 expression levels were checked by immunoprecipitate technique.Results UV radiation can induce p53 gene over-expression and the p53 over-expression can last 360 minutes in this study after UV radiation.Conclusion The results indicate that extracellular DNA damage agents are supposed to induce p53 gene over-expression,and that p53 over-expression is the functional reaction to cellular DNA damage.