慢性乙型肝炎病情再活动时血清HBV DNA水平的变化

Changes of Serum HBV DNA level of Disease Reactivations in Patients with Chronic Hepatitis B

【目的】研究慢性乙型肝炎患者HBV复制水平与病情活动关系。【方法】采用荧光标记定量PCR的方法,测定一组急性发作的慢性乙型肝炎患者(其中HBeAg阳性组38例,抗HBe阳性组22例)病情活动期和恢复期的血清HBVDNA含量。【结果】血清HBVDNA含量在活动期明显高于恢复期(P<005)。血清HBVDNA含量与丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)及总胆红素(TBLLI)之间无相关性。【结论】HBVDNA复制水平与慢性乙型肝炎的活动密切相关。

Objective We study the changes of HBV DNA serum level in acute hepatic exacerbation of the disease in chronic hepatitis B patients. Methods The serum HBV DNA concentration in 60 patients with acute exacerbation (AE) of chronic hepatitis B were tested by the quantitative PCR using the AmpliSensor assay. Results In 60 patients with CHB (38 HBeAg positive and 22 anti HBe positive), levers of the serum HBV DNA in sera were higher during acute attacks than the one during recovery (P<0 05); No correlation were found among the levels of HBV DNA, alanine aminotransferase(ALT), aspartate aminotransferase (AST) and total bilirubin. Conclusions This investigation revealed there was significant correlation between the increasing levels of serum HBV DNA and the reactivations of patients in chronic hepatitis B. The increases in viral replication and subsequent host immune response to virus infected hepatocytes play an important role in initiating reactivation of patients with CHB.