Tat和TAR对人免疫缺陷病毒HIV-1基因表达的调控研究

Functional analysis of TAT protein and TAR element in the regulation of HIV-1 gene expression

目的为了寻找能够阻断HIV-1复制的方法,探索控制HIV-1蔓延的第二代基因治疗的新途径。方法采用自身引物一模板PCR得到了含有TAR核心序列(+11到+47)的多聚TAR-Core DNA同向串联体。结果所有这些抗性基因策略均能对病毒的复制产生较好的抑制作用。结论 TAT蛋白序列中氨基酸的替换使活性改变,与其三维结构的变化间有一定的相关性。

Objective In order to find the way to blockade the HIV replication and control the HIV - spreading, the second generation gene therapy was investigated. Methods By using a self- primer template PCR we generated the multimerized TAT DNAs containing different tandem copies of TAR core element ( + 11 to +17). Results The replicated HIV - 1 was inhibited by all the anti - HIV strategies tested. Conclusion TAT protein sequences of amino acid the activity was changed, the alternation and with 3 - dimentional structural variation has a definitive relationship