MicroRNA-146a抑制胶质瘤细胞增殖的研究

MicroRNA-146a Inhibits the Proliferation of Glioma Cell

目的:检测胶质瘤中miR-146a的表达水平,并研究miR-146a对胶质瘤细胞增殖的影响。方法:应用实时定量PCR的方法检测胶质瘤组织和癌旁组织中miR-146a的表达水平,采用脂质体细胞转染miRNA模拟物的方式过表达miR-146a,MTT法检测转染后细胞的增殖率,利用在线软件targetScan预测miRNA可能的靶基因。结果:miR-146a在胶质瘤组织中表达明显降低(P<0.01),相对表达水平为癌旁组织的35%,细胞转染miR-146a模拟物后,miR-146a表达明显增加,癌细胞增殖率明显降低(P<0.01),仅为原细胞的47%。Notch1基因是miR-146a影响胶质瘤细胞增殖活力的可能靶基因。结论:miR-146a可能通过抑制Notch1基因的表达调控胶质瘤细胞的增殖。

Objective： To investigate the abnormally expression and effect of miR-146a on proliferation in glioma cancer.Methods： Real-Time PCR assay was used to determine the abnormally expression of miR-146a in tumor-adjacent tissues and glioma tissues.The activity of miR-146a was up-regulated by the method of lipofection of the mimic of miR-146a.Real-time PCR was also used to observe the expression level of miR-146a.Cell viability analysis was done by MTT.The targeted genes of miR-146a in glioma were scanned by the online targeted gene prediction software.Results： miR-146a was detected down-regulated in glioma tissues,with 35% for tumor-adja-cent tissues（p0.01）.The cell viability was decreased after miR-146a was up-regulated in glioma cell by miR-146a-mimic transfection.Notch1 was the potential targeted gene of miR-146a in glioma.Conclusions： miR-146a could affect the proliferation of glioma cell by in-hibiting the Notch1 expression.