AOA对牡丹切花花期叶片和花瓣生理的影响

Effect of AOA on Leaves and Petals Physiology in Cut Tree Peony

以不同浓度(0、0.25、0.50、0.75 mmol/L)的氨基氧乙酸(AOA)对待放牡丹"赵粉"进行处理,测定花期叶片、花瓣的生理生化变化。结果表明,在牡丹切花的整个花期中,叶片的叶绿素含量和可溶性蛋白含量均在花开初期上升,在盛花期达到峰值后下降。经AOA处理后,与对照组相比,其花期花瓣中可溶性糖含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均升高,花瓣中超氧阴离子(O2-.)产生速率、花瓣浸出液相对电导率(REC)在整个花期均降低;AOA使牡丹花期花瓣的ACC合成酶(ACS)酶带推迟表达,并使原有酶带减弱。其中0.25 mmol/L AOA保鲜效果最佳。

The cut tree peony ＂zhaofen＂ was treated with different concentrations（0,0.25,0.50,0.75 mmol/L） of aminooxyacetate（AOA） and physiological indexes in leaves and petals were determined during florescence in this paper.The results indicated that,during the cut peony whole in flowering,chlorophyll and soluble protein content in leaves rose in bloom early,then declined after full-bloom stage peak.After AOA treatment,compared with CK,soluble sugar content,superoxide dismutase（SOD） and peroxidase（POD） activity in petals improved,superoxide anion（O2-·） production rate and relative electronical conductivity in petals decreased.ACC synthase（ACS） was delayed expression,and make the original enzyme belt weak.In all treatments,0.25 mmol/L AOA showed the best fresh-keeping effect.