消瘀通络散对乳腺增生大鼠乳腺组织形态与内分泌的影响

Effects of Xiaoyu Tongluo San on breast morphology and endocrine in rats with hyperplasia of mammary glands

目的:观察自拟消瘀通络散对乳腺增生大鼠乳头直径、高度,血清E2/P、PRL、T水平以及乳腺组织形态的影响,探讨其治疗乳腺增生的作用机理。方法:将40只Wistar大鼠随机分为正常组、模型组、中药组和西药组4组。采用己烯雌酚联合黄体酮肌注的方法进行造模。中药组给予自拟消瘀通络散50mg/d,西药组予三苯氧胺1.8mg/kg,均灌胃治疗,1次/d,共30d。于治疗前后测量各组大鼠第2对乳头直径、高度,测定血清E2、P、PRL、T水平,计算E2/P数值,取大鼠第2对乳房常规HE染色,光镜下观察组织形态表现。结果:治疗后乳头直径、高度比较,中药组和西药组均低于模型组(P<0.05);2组之间比较,中药组低于西药组(P<0.05)。血清各激素水平比较,中药组和西药组血清E2/P、PRL、T含量均低于模型组(P<0.05);中药组与西药组相比无显著性差异(P>0.05)。病理学比较,中药组和西药组大鼠乳腺组织病理表现相似,与模型组比较改善明显,与正常组相近。结论:自拟消瘀通络散具有缩小模型大鼠乳头直径、高度,改善内分泌紊乱状态和乳腺组织病理形态的作用。

Objective： To observe the effect of Xiaoyu tongluo san on the second pair of breast nipple diameter,height and serum E2/P ratio,PRL,T levels、breast tissue of the Mammary Gland Hyperplasia rats model,discuss the therapeutic mechanism of HMG.Methods： 40 Wistar rats were divided into 4 groups randomly：normal group,model group,TCM group and western medicine group.Combined diethylstilbestrol with progesterone by intramuscular injection to copy breast Hyperplasia rats model.TCM group were treated with self-Xiaoyu tongluo san 50mg/d,while western medicine group were treated with TAM 1.8mg/kg,gavage was both applied,1 time/d,30 days totally.Detected the second pair of breast nipple diameter,height and serum E2/P ratio,PRL,T levels before and after treatment,calculate E2/P ratio,The second pair of nipple were removed aseptically,stained HE,breast forms was observed under microscope.Results： After treatment,comparision of the second breast nipple diameter and height： TCM group and western medicine group were lower than model group（P0.05）;TCM group were lower than western medicine group（P0.05）.Comparision of serum levels of the hormone： TCM group and western medicine group were lower than model group（P0.05）,compared TCM group with western medicine group,the difference was not statistically significant（P0.05）.Comparision of breast tissue pathological forms： The pathological forms in TCM group were similar to those in TAM group,but they were both better than that in model group,and similar to the normal group.Conclusion： The self-Xiaoyu tongluo san has effects on reducing nipple diameter,height and regulating the dysfuction of serum E2/P ratio,PRL,T and improve the breast tissue pathological forms of Mammary Gland Hyperplasia rats.