基质金属蛋白酶-9、环氧化酶-2、E-钙粘素在甲状腺微小乳头状癌中的表达及其与颈部淋巴结转移的关系

Expression of MMP-9, COX-2 and E-cadherin in papillary thyroid microcarcinoma and its rela- tionship with cervical lymph node metastasis

目的探讨基质金属蛋白酶-9（MMP-9）、环氧化酶-2（COX-2）和E-钙粘素（E—cadherin）在甲状腺微小乳头状癌（PTMC）组织中的表达及其与颈淋巴结转移的关系。方法应用免疫组织化学方法检测47例PTMC组织中的MMP-9、COX-2、E-cadherin的表达情况，并进行颈部淋巴结转移相关性分析。结果PTMC组织中MMP-9、COX-2、E—cadherin表达阳性率分别为44．7％（21／47）、57．5％（27／47）和61．7％（29／47），其中颈部淋巴结转移组MMP-9、COX-2、E—cadherin的表达阳性率分别为69．2％（9／13）、84．6％（11／13）和30．8％（4／13），无颈淋巴结转移组分别为35．3％（12／34）、47．1％（16／34）和73．5％（25／34），两组比较差异均有统计学意义（P〈0．05）。PTMC组织中MMP-9、COX-2表达与淋巴结转移呈正相关，E—cadherin表达与淋巴结转移呈负相关。结论PTMC组织中MMP-9、COX-2、E—cadherin的表达与颈部淋巴结转移有关，检测其表达对判断临床进展以及推测预后有一定的参考价值。

Objective To investigate the expressions of MMP-9, COX-2 and E-cadherin in papillary thyroid microeareinoma（PTMC） and its relationship with cervical lymph node metastasis. Methods Forty-seven papillary thyroid microcarcinoma （PTMC） specimens were stained for MMP-9, COX-2 and E-cadherin by immunohistochemical technique. Correlation of cervical lymph node metastasis with expression of MMP-9, COX-2 and E- cadherin was analyzed. Results The positive expression rates of MMP-9, COX-2 and E-cadherin in PT- MC were 44.7 % （21/47 ）, 57.5 % （ 27/47 ） and 61.7 % （ 29/47 ）, respectively. The positive expression rates of patients with cervical lymph node metastasis were 69. 2% （9/13）, 84.6% （ 11/13 ） and 30. 8% （4/13）, while were 35.3% （12/34）, 47.1% （16/34）and 73.5% （25/34） in patients without lymphnode metastasis. There were significant differences in the expression of MMP-9, COX-2 and E-cadherin in PTMC with and without cervical lymph metastasis （P 〈 0. 05 ）. The expressions of MMP-9 and COX-2 in papillary thyroid microcarcinoma （PTMC） were positively related with cervical lymph node metastases, while the expressions of E-cadherin in papillary thyroid microcarcinoma （PTMC） was negatively related with cervical lymph node metastases. Conclusions The expressions of MMP-9, COX-2, E-cadherin in papillary thyroid microcarcinoma were related to cervical lymph node metastasis, and could be helpful indicators for judging clinical progress and estimating prognosis.