芹灵冲剂在2215细胞培养内对HBsAg,HBeAg的抑制作用

Inhibition of Qinling Chongji on HBsAg and HBeAg secretion in cultured cell line 2215

目的 在乙型肝炎病毒（ＨＢＶ）基因的人肝癌细胞系（ＨｅｐＧ２的２２１５细胞培养中，观察芹灵冲剂（Ｑｉｎｌｉｎｇ Ｃｈｏｎｇｌｉ，ＱＬ）对细胞的毒性和对乙型肝炎病毒表面抗原（ＨＢｓＡＧ）和ｅ抗原（ＨＢｅＡｇ）的抑制效果。方法 （１）２２１５细胞培养。将配制（１×１０＾５个／ｍＬ）接种细胞培养板，９６孔板每孔０．１２ｍＬ，２４孔板每孔１ｍＬ，３７℃细胞毒笥试验：ＱＬ１２ｇ／Ｌ用２液２倍稀释成６个浓度，

AIM To study the inhibiting effect of Qinling Chongji ( QL ) on HBsAg and HBeAg in the cultured hepatocarcinoma cell line 2215 (Hep G2) which was genetransfered with hepatitis·B virus (HBV) and its toxicity.METHODS Twenty-four hours after 2215 cells vaccination, the varied concentrations of QL were added to culture fluid, and exchanged the drugs every four days until to 8 d and 12 d. The cell alterations were observed under microscopy in order to evaluate the toxicity of QL. After treated with QL for 8 d and 12 d, the culture fluids were collected and the content of HBeAg and HBsAg was determined by using radioimmunoassay kits. RESULTS QL in nontoxic concentration significantly inhibited the secretion of HBsAg and HBeAg in cell line 2215 after 8 d and 12 d treatment. The mean inhibiting rate of QL (4000 μg·mL-1) on HBeAg was 51.57%±2.14% (8d) and 65.06%±1.94% (12 d), [ IC50 = (8 d) 3090.19 μg·mL-1±772.40 μg·mL-1 and (12 d) 907.13 μg·mL-1±93.57 μg·mL-1]; and was (8 d) 55.04% ±3.88% and (12d) 61.39%±0.98%, (IC50) = (8d) 2993.89 μg·mL-1±768.66 μg·mL-1 and (12 d) 1744.44 μg·mL-1±214.08 μg·mL-1 respectively. CONCLUSION The results show that QL can inhibit the secretion of HBsAg and HBeAg in cell line 2215 and has no toxicity at high concentration.